Biotechnology- Principles and Processes Question 4
4. The specific palindromic sequence which is recognized by EcoRl is
(a) $5^{\prime}$ - GGAACC - $3^{\prime}$ $3^{\prime}$ - CCTTGG - $5^{\prime}$
(b) $5^{\prime}$ - CTTAAG - $3^{\prime}$ $3^{\prime}$ - GAATTC - $5^{\prime}$
(c) $5^{\prime}$ - GGATCC - $3^{\prime}$ $3^{\prime}$ - CCTAGG - $5^{\prime}$
(d) $5^{\prime}$ - GAATTC - $3^{\prime}$ $3^{\prime}$ - CTTAAG - $5^{\prime}$
Show Answer
Answer : d
Hints & Solutions
(d) The correct option is (d) because the specific palindromic sequence which is recognised by $E c o R I$ is
5’ - GAATTC - 3'
3’ - CTTAAG - 5'
EcoRI creates 4 nucleotide sticky ends with 5 ’ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.
