Biotechnology- Principles and Processes Question 4

4. The specific palindromic sequence which is recognized by EcoRl is

(a) 5 - GGAACC - 3 3 - CCTTGG - 5

(b) 5 - CTTAAG - 3 3 - GAATTC - 5

(c) 5 - GGATCC - 3 3 - CCTAGG - 5

(d) 5 - GAATTC - 3 3 - CTTAAG - 5

Show Answer

Answer : d

Hints & Solutions

(d) The correct option is (d) because the specific palindromic sequence which is recognised by EcoRI is

5’ - GAATTC - 3'

3’ - CTTAAG - 5'

EcoRI creates 4 nucleotide sticky ends with 5 ’ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.



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