Biotechnology- Principles and Processes Question 4
4. The specific palindromic sequence which is recognized by EcoRl is
(a)
(b)
(c)
(d)
Show Answer
Answer : d
Hints & Solutions
(d) The correct option is (d) because the specific palindromic sequence which is recognised by
5’ - GAATTC - 3'
3’ - CTTAAG - 5'
EcoRI creates 4 nucleotide sticky ends with 5 ’ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.