Biotechnology- Principles and Processes Question 4
4. The specific palindromic sequence which is recognized by EcoRl is
(a) 5’ - GGAACC - 3'
(b) 5’ - CTTAAG - 3'
(c) 5’ - GGATCC - 3'
(d) 5’ - GAATTC - 3'
Show Answer
Answer : d
Hints & Solutions
(d) The correct option is
(d) because the specific palindromic sequence which is recognised by $E c o R I$ is
5’ - GAATTC - 3'
3’ - CTTAAG - 5 ,
EcoRI creates 4 nucleotide sticky ends with 5 ’ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.
