Biotechnology- Principles and Processes Question 30
30. Which one of the following represents a palindromic sequence in DNA?
(a) $5^{\prime}$ - GAATTC - $3^{\prime}$ $3^{\prime}$ - CTTAAG - $5^{\prime}$
(b) $5^{\prime}$ - CCAATG - $3^{\prime}$ $3^{\prime}$ - GAATCC - $5^{\prime}$
(c) $5^{\prime}$ - CATTAG - $3^{\prime}$ $3^{\prime}$ - GATAAC - $5^{\prime}$
(d) $5^{\prime}$ - GATACC - $3^{\prime}$ $3^{\prime}$ - CCTAAG - $5^{\prime}$
Show Answer
Answer : a
Hints & Solutions
(a) A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read $5^{\prime}$ (five-prime) to $3^{\prime}$ (three prime) on one strand or $5^{\prime}$ to $3^{\prime}$ on the complementary strand with which it forms a double helix.
$5^{\prime}$ - GAATTC - $3^{\prime}$
$3^{\prime}$ - CTTAAG - $5^{\prime}$
It is a palindromic sequence of DNA cut by restriction enzyme EcoRI.
