Biomolecules Question 78
78. The introduction of t-DNA into plants involves:
[2015 RS]
(a) Altering the $pH$ of the soil, then heat shocking the plants
(b) Exposing the plants to cold for a brief period
(c) Allowing the plant roots to stand in water
(d) Infection of the plant by Agrobacterium tumefaciens
Show Answer
Answer : d
Hints & Solutions
(d) Agrobacterium tumefaciens introduces t-DNA into the plant. Agrobacterium tumefaciens is a soil bacterium that has the ability to infect plant cells and transfer a defined sequence of their DNA to the plant cell by infection and a causative agent of crown gall disease. Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumorinducing) plasmid (140-235 kb).
