Biomolecules Question 78

78. The introduction of t-DNA into plants involves:

[2015 RS]

(a) Altering the $pH$ of the soil, then heat shocking the plants

(b) Exposing the plants to cold for a brief period

(c) Allowing the plant roots to stand in water

(d) Infection of the plant by Agrobacterium tumefaciens

Show Answer

Answer : d

Hints & Solutions

(d) Agrobacterium tumefaciens introduces t-DNA into the plant. Agrobacterium tumefaciens is a soil bacterium that has the ability to infect plant cells and transfer a defined sequence of their DNA to the plant cell by infection and a causative agent of crown gall disease. Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumorinducing) plasmid (140-235 kb).