Reproduction:Reproduction In Organisms – 3

Plant Tissue Culture

graph TD; A[Plant Tissue Culture] A --> B[Definition] A --> C[Types] A --> D[Applications] A --> E[Advantages] A --> F[Disadvantages] B --> G[Placeholder 1] B --> H[Placeholder 2] C --> I[Placeholder 3] C --> J[Placeholder 4] D --> K[Placeholder 5] D --> L[Placeholder 6] E --> M[Placeholder 7] E --> N[Placeholder 8] F --> O[Placeholder 9] F --> P[Placeholder 10]

Instruments

graph TD; Instruments-->Microscope; Instruments-->Centrifuge; Instruments-->Spectrophotometer; Instruments-->PCR_Machine[PCR Machine]; Instruments-->Autoclave; Microscope-->Compound_Microscope[Compound Microscope]; Microscope-->Electron_Microscope[Electron Microscope]; Centrifuge-->High_Speed_Centrifuge[High Speed Centrifuge]; Centrifuge-->Ultracentrifuge; Spectrophotometer-->UV_Spectrophotometer[UV Spectrophotometer]; Spectrophotometer-->Infrared_Spectrophotometer[Infrared Spectrophotometer]; PCR_Machine-->Thermal_Cycler[Thermal Cycler]; Autoclave-->Steam_Autoclave[Steam Autoclave]; Autoclave-->Gas_Autoclave[Gas Autoclave];

Procedure Involved In Plant Tissue Culture

graph TB A[Plant Tissue Culture] A --> B[Selection of Plant Material] A --> C[Preparation of Culture Medium] A --> D[Sterilization] A --> E[Inoculation] A --> F[Incubation] A --> G[Subculturing] A --> H[Hardening]

Preparation Of Culture Media

graph TB A[Preparation Of Culture Media] A --> B[Types of Culture Media] B --> B1[Selective Media] B --> B2[Differential Media] B --> B3[Enriched Media] A --> C[Components of Culture Media] C --> C1[Peptones] C --> C2[Sugars] C --> C3[Salts] C --> C4[Water] A --> D[Preparation Process] D --> D1[Sterilization] D --> D2[Inoculation] D --> D3[Incubation]

The Culture Media And Its Streilization

graph TD; A[The Culture Media And Its Sterilization]; B[Types of Culture Media]; C[Preparation of Culture Media]; D[Sterilization Methods]; E[Quality Control]; A-->B; A-->C; A-->D; A-->E; B-->F[Selective Media]; B-->G[Differential Media]; B-->H[Enriched Media]; C-->I[Ingredients]; C-->J[Procedure]; D-->K[Autoclaving]; D-->L[Filtration]; D-->M[Radiation]; E-->N[Testing for Sterility]; E-->O[Media Performance Test];

Selection And Surface Sterilisation Of The Explant

graph TD; A[Selection And Surface Sterilisation Of The Explant]; B[Selection of Explant]; C[Surface Sterilisation]; D[Types of Explants]; E[Methods of Sterilisation]; F[Importance of Sterilisation]; A-->B; A-->C; B-->D; C-->E; C-->F;

Inoculation And Callus Formation

graph TD; A[Inoculation And Callus Formation]; B[Inoculation]; C[Callus Formation]; D[Methods of Inoculation]; E[Process of Callus Formation]; F[Applications of Inoculation]; G[Applications of Callus Formation]; A-->B; A-->C; B-->D; B-->F; C-->E; C-->G;

Incubation For The Development Of Root And Shoot In The Plantlet

graph TB A[Incubation For The Development Of Root And Shoot In The Plantlet] A --> B[Root Development] B --> B1[Root Initiation] B --> B2[Root Elongation] B --> B3[Root Differentiation] A --> C[Shoot Development] C --> C1[Shoot Initiation] C --> C2[Shoot Elongation] C --> C3[Shoot Differentiation]

Acclimatization And Hardening

graph TD; A[Acclimatization And Hardening]; B[Subtopic 1]; C[Subtopic 2]; D[Subtopic 3]; E[Subtopic 4]; A-->B; A-->C; A-->D; A-->E; B-->F[Sub-subtopic 1]; B-->G[Sub-subtopic 2]; C-->H[Sub-subtopic 3]; C-->I[Sub-subtopic 4]; D-->J[Sub-subtopic 5]; D-->K[Sub-subtopic 6]; E-->L[Sub-subtopic 7]; E-->M[Sub-subtopic 8];