Plant Tissue Culture
graph TD;
A[Plant Tissue Culture]
A --> B[Definition]
A --> C[Types]
A --> D[Applications]
A --> E[Advantages]
A --> F[Disadvantages]
B --> G[Placeholder 1]
B --> H[Placeholder 2]
C --> I[Placeholder 3]
C --> J[Placeholder 4]
D --> K[Placeholder 5]
D --> L[Placeholder 6]
E --> M[Placeholder 7]
E --> N[Placeholder 8]
F --> O[Placeholder 9]
F --> P[Placeholder 10]
Instruments
graph TD;
Instruments-->Microscope;
Instruments-->Centrifuge;
Instruments-->Spectrophotometer;
Instruments-->PCR_Machine[PCR Machine];
Instruments-->Autoclave;
Microscope-->Compound_Microscope[Compound Microscope];
Microscope-->Electron_Microscope[Electron Microscope];
Centrifuge-->High_Speed_Centrifuge[High Speed Centrifuge];
Centrifuge-->Ultracentrifuge;
Spectrophotometer-->UV_Spectrophotometer[UV Spectrophotometer];
Spectrophotometer-->Infrared_Spectrophotometer[Infrared Spectrophotometer];
PCR_Machine-->Thermal_Cycler[Thermal Cycler];
Autoclave-->Steam_Autoclave[Steam Autoclave];
Autoclave-->Gas_Autoclave[Gas Autoclave];
Procedure Involved In Plant Tissue Culture
graph TB
A[Plant Tissue Culture]
A --> B[Selection of Plant Material]
A --> C[Preparation of Culture Medium]
A --> D[Sterilization]
A --> E[Inoculation]
A --> F[Incubation]
A --> G[Subculturing]
A --> H[Hardening]
graph TB
A[Preparation Of Culture Media]
A --> B[Types of Culture Media]
B --> B1[Selective Media]
B --> B2[Differential Media]
B --> B3[Enriched Media]
A --> C[Components of Culture Media]
C --> C1[Peptones]
C --> C2[Sugars]
C --> C3[Salts]
C --> C4[Water]
A --> D[Preparation Process]
D --> D1[Sterilization]
D --> D2[Inoculation]
D --> D3[Incubation]
graph TD;
A[The Culture Media And Its Sterilization];
B[Types of Culture Media];
C[Preparation of Culture Media];
D[Sterilization Methods];
E[Quality Control];
A-->B;
A-->C;
A-->D;
A-->E;
B-->F[Selective Media];
B-->G[Differential Media];
B-->H[Enriched Media];
C-->I[Ingredients];
C-->J[Procedure];
D-->K[Autoclaving];
D-->L[Filtration];
D-->M[Radiation];
E-->N[Testing for Sterility];
E-->O[Media Performance Test];
Selection And Surface Sterilisation Of The Explant
graph TD;
A[Selection And Surface Sterilisation Of The Explant];
B[Selection of Explant];
C[Surface Sterilisation];
D[Types of Explants];
E[Methods of Sterilisation];
F[Importance of Sterilisation];
A-->B;
A-->C;
B-->D;
C-->E;
C-->F;
graph TD;
A[Inoculation And Callus Formation];
B[Inoculation];
C[Callus Formation];
D[Methods of Inoculation];
E[Process of Callus Formation];
F[Applications of Inoculation];
G[Applications of Callus Formation];
A-->B;
A-->C;
B-->D;
B-->F;
C-->E;
C-->G;
Incubation For The Development Of Root And Shoot In The Plantlet
graph TB
A[Incubation For The Development Of Root And Shoot In The Plantlet]
A --> B[Root Development]
B --> B1[Root Initiation]
B --> B2[Root Elongation]
B --> B3[Root Differentiation]
A --> C[Shoot Development]
C --> C1[Shoot Initiation]
C --> C2[Shoot Elongation]
C --> C3[Shoot Differentiation]
Acclimatization And Hardening
graph TD;
A[Acclimatization And Hardening];
B[Subtopic 1];
C[Subtopic 2];
D[Subtopic 3];
E[Subtopic 4];
A-->B;
A-->C;
A-->D;
A-->E;
B-->F[Sub-subtopic 1];
B-->G[Sub-subtopic 2];
C-->H[Sub-subtopic 3];
C-->I[Sub-subtopic 4];
D-->J[Sub-subtopic 5];
D-->K[Sub-subtopic 6];
E-->L[Sub-subtopic 7];
E-->M[Sub-subtopic 8];