Shortcut Methods
Numerical Values for Microbes in Human Welfare
1. Curd Formation:
- Lactobacillus converts 1 gram of lactose into 0.5 grams of lactic acid in 1-2 hours at 37°C.
2. Nitrogen Cycle:
- Nitrobacter converts 10-20% of nitrite into nitrates during nitrification.
3. Biogas Production:
- Biogas typically contains 55-75% methane, 25-45% carbon dioxide, and traces of other gases.
4. Antibiotics:
- Penicillin extracted from Penicillium notatum in 1928 had an antibacterial activity of around 16 units per milliliter.
5. Vaccine Production:
- One milliliter of Jonas Salk’s polio vaccine contains approximately 13,500 million killed poliovirus particles.
6. Sewage Treatment:
- In activated sludge treatment, 25-35% of wastewater is recycled as return sludge, while the rest undergoes biological treatment.
7. Microbes in Food:
- Fermentation of soybeans by Rhizopus oligosporus or Aspergillus oryzae produces tempe with a protein content of around 20-25%.
8. Biological Control Agents:
- Neem tree (Azadirachta indica) produces azadirachtin with an LD50 (lethal dose to 50% of the target pest population) of approximately 3-5 ppm.
9. Bioremediation:
- Microbes can degrade oil spills at a rate of approximately 0.1-0.5% of hydrocarbons per day.
10. Microbiology Techniques:
- Serial dilutions in microbiology are commonly done in 1:10 or 1:100 ratios.
- Colony counting or spectrophotometry is used to estimate the number of microbes in a sample.