Biotechnology Principles And Processes Question 2

Question: Which of the following is not a cloning vector?

(1) Probe

(2) BAC

(3) YAC

(4) pBR322.

Show Answer

Answer: (1) Probe

Explaination-

BAC (Bacterial artificial chromosome)- useful for studying large viruses because the DNAs of these viruses are too large to be cloned in individual plasmids

YAC (Yeast artificial chromosomes)- genetically engineered chromosomes derived from the DNA of the yeast.

pBR322- a commonly used plasmid cloning vector in E. coli

Probe- a single-stranded sequence of DNA or RNA that is used to identify specific sequences of DNA or RNA