NEET Solved Paper 2018 Question 11

Question: The correct order of steps in Polymerase Chain Reaction (PCR) is [NEET - 2018]

Options:

A) Denaturation, Extension, Annealing

B) Annealing, Extension, Denaturation

C) Extension, Denaturation, Annealing

D) Denaturation, Annealing, Extension

Show Answer

Answer:

Correct Answer: D

Solution:

  • This technique is used for making multiple copies of gene (or DNA) of interest in vitro. Each cycle has three steps (i) Denaturation (ii) Primer annealing (iii) Extension of primer