Sexual Reproduction in Flowering Plants Question 94
94. The viability of seeds is tested by
(a) Safranine
(b) 2,6 dichlorophenol indophenols
(c) 2,3, 5 triphenyl tetrazolium chloride
(d) DMSO
Show Answer
Answer : c
Hints & Solutions
(c) Dehydrogenase enzymes present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water insoluble compound. This reaction occurs in or near living cells which are releasing hydrogen in respiration processes. Viable tissues produce a normal red-colour, weak living tissue produce an abnormal colour. Dead tissues do not stain, remaining usually white.
