Biotechnology- Principles and Processes Question 55

55. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :

[2012]

(a) Silver or Platinum

(b) Platinum or Zinc

(c) Silicon or Platinum

(d) Gold or Tungsten

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Answer : d

Hints & Solutions

(d) For gene transfer into the host cell without using vector microparticles made of tungsten and gold coated with foreign DNA are bombarded into target cells at a very high velocity. The metal particles, usually gold or tungsten are used for coating in gene gun and are accelerated to high speed by the rapid release of high pressure helium in the gene gun into the target cells.



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