Biotechnology- Principles and Processes Question 53
53. PCR and Restriction Fragment Length Polymorphism are the methods for :
[2012]
(a) Study of enzymes
(b) Genetic transformation
(c) DNA sequencing
(d) Genetic Fingerprinting
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Answer : d
Hints & Solutions
(d) PCR and RFLP are the methods used for genetic fingerprinting. DNA fragmentation plays an important part in forensics, especially that of DNA profiling. Restriction fragment length polymorphism (RFLP) analyses the variable lengths of DNA fragments which are formed from the digestion of a DNA sample with a restriction endonuclease. They are then hybridized with DNA probes that bind to a complementary DNA sequence in the sample. In polymerase chain reaction (PCR), DNA sample is amplified from a single copy to millions of copies of DNA. It used to amplify a specific region of a DNA strand. Most PCR methods amplify DNA fragments of between 0.1 and 10 kilo base pairs (kb), although some techniques allow amplification of fragments up to $40 kb$ in size.
