Biotechnology- Principles and Processes Question 52
52. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :
[NEET 2013]
(a) Polymerase chain reaction
(b) Electrophoresis
(c) Restriction mapping
(d) Centrifugation
Show Answer
Answer : b
Hints & Solutions
(b) In a chemical reaction DNA fragments generated by restriction endonucleases, can be separated by gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate according to their size through sieving effect provided by matrix.
