Biotechnology- Principles and Processes Question 39
39. The linking of antibiotic resistance gene with the plasmid vector became possible with
[2008]
(a) DNA ligase
(b) Endonucleases
(c) DNA polymerase
(d) Exonucleases
Show Answer
Answer : a
Hints & Solutions
(a) The linking of antibiotic resistance gene with the plasmid vector became possible with DNA ligase. DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology as it ensures that the foreign DNA is bound to the plasmid into which it is incorporated.
DNA ligase is an enzyme which can connect two strands of DNA together by forming a bond between the phosphate group of one strand and the deoxyribose group on another.
