Biotechnology- Principles and Processes Question 26
26. The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bateria because of: [NEET 2013]
(a) Insertional inactivate of alphagalactosidase in non-recombinant bacteria
(b) Insertional inactivation of alphagalactosidase in recombinant bacteria
(c) Inactivation of glycosidase enzyme in recombinant bacteria
(d) Non-recombinant bacteria containing beta-galactosidase
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Answer : d
Hints & Solutions
(d) Alternative selectable markers have been developed which differentiate recombinant from non-recombinants on the basis of their ability to produce colour in the presence of chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme