Biotechnology- Principles and Processes Question 14
14. The correct order of steps in Polymerase Chain Reaction (PCR) is
(a) Extension, Denaturation, Annealing
(b) Annealing, Extension, Denaturation
(c) Denaturation, Annealing, Extension
(d) Denaturation, Extension, Annealing
Show Answer
Answer : c
Hints & Solutions
(c) PCR is based on three simple steps required for any DNA synthesis reaction: (i) denaturation of the template into single strands; (ii) annealing of primers to each original strand for new strand synthesis; and (iii) extension of the new DNA strands from the primers.
