Biotechnology and Its Applications Question 27
27. Match the following columns and select the correct option.
| Column-I | Column-II | ||
|---|---|---|---|
| (a) | Bt cotton | (i) | Gene therapy |
| (b) | Adenosine deaminase deficiency |
(ii) | Cellular defence |
| (c) | RNAi | (iii) | Detection of HIV infection |
| (d) | PCR | (iv) | Bacillus thuringiensis |
| (A) | (B) | (C) | (D) | |
|---|---|---|---|---|
| (a) | (iii) | (ii) | (i) | (iv) |
| (b) | (ii) | (iii) | (iv) | (i) |
| (c) | (i) | (ii) | (iii) | (iv) |
| (d) | (iv) | (i) | (ii) | (iii) |
Show Answer
Answer : d
Hints & Solutions
(d) The correct option is (d) because in Bt cotton the specific Bt toxin gene was isolated from Bacillus thuringiensis. The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. RNAi (RNA interference) takes place in all eukaryotic organisms as a method of cellular defense. PCR is now routinely used to detect HIV in suspected AIDS patients.
The PCR helps to detect the low amount of DNA by amplifying that piece of DNA to form many copies. The amplified DNA can be then easily detected by using other testing procedures. This technique is used to detect infection at the early stage by amplifying the DNA of microbe.
