Breathing And Exchange Of Gases
Breathing And Exchange Of Gases
As you have read earlier, oxygen (O2) is utilised by the organisms to indirectly break down simple molecules like glucose, amino acids, fatty acids, etc., to derive energy to perform various activities. Carbon dioxide (CO2) which is harmful is also released during the above catabolic reactions. It is, therefore, evident that O2 has to be continuously provided to the cells and CO2 produced by the cells have to be released out. This process of exchange of O2 from the atmosphere with CO2 produced by the cells is called breathing, commonly known as respiration. Place your hands on your chest; you can feel the chest moving up and down. You know that it is due to breathing. How do we breathe? The respiratory organs and the mechanism of breathing are described in the following sections of this chapter.
17.1 RESPIRATORY ORGANS
Mechanisms of breathing vary among different groups of animals depending mainly on their habitats and levels of organisation. Lower invertebrates like sponges, coelenterates, flatworms, etc., exchange O2 with CO2 by simple diffusion over their entire body surface. Earthworms use their moist cuticle and insects have a network of tubes (tracheal tubes) to transport atmospheric air within the body. Special vascularised structures called gills (branchial respiration) are used by most of the aquatic arthropods and molluscs whereas vascularised bags called lungs (pulmonary respiration) are used by the terrestrial forms for the exchange of gases. Among vertebrates, fishes use gills whereas amphibians, reptiles, birds and mammals respire through lungs. Amphibians like frogs can respire through their moist skin (cutaneous respiration) also.
17.1.1 Human Respiratory System
We have a pair of external nostrils opening out above the upper lips. It leads to a nasal chamber through the nasal passage. The nasal chamber opens into the pharynx, a portion of which is the common passage for food and air. The pharynx opens through the larynx region into the trachea. Larynx is a cartilaginous box which helps in sound production and hence called the sound box. During swallowing glottis can be covered by a thin elastic cartilaginous flap called epiglottis to prevent the entry of food into the larynx. Trachea is a straight tube extending up to the mid-thoracic cavity, which divides at the level of 5th thoracic vertebra into a right and left primary bronchi. Each bronchi undergoes repeated divisions to form the secondary and tertiary bronchi and bronchioles ending up in very thin terminal bronchioles. The tracheae, primary, secondary and tertiary bronchi, and initial bronchioles are supported by incomplete cartilaginous rings. Each terminal bronchiole gives rise to a number of very thin, irregular-walled and vascularised bag-like structures called alveoli. The branching network of bronchi, bronchioles and alveoli comprise the lungs (Figure 17.1). We have two lungs which are covered by a double layered pleura, with pleural fluid between them. It reduces friction on the lung-surface. The outer pleural membrane is in close contact with the thoracic
lining whereas the inner pleural membrane is in contact with the lung surface. The part starting with the external nostrils up to the terminal bronchioles constitute the conducting part whereas the alveoli and their ducts form the respiratory or exchange part of the respiratory system. The conducting part transports the atmospheric air to the alveoli, clears it from foreign particles, humidifies and also brings the air to body temperature. Exchange part is the site of actual diffusion of O2 and CO2 between blood and atmospheric air.
The lungs are situated in the thoracic chamber which is anatomically an air-tight chamber. The thoracic chamber is formed dorsally by the vertebral column, ventrally by the sternum, laterally by the ribs and on the lower side by the dome-shaped diaphragm. The anatomical setup of lungs in thorax is such that any change in the volume of the thoracic cavity will be reflected in the lung (pulmonary) cavity. Such an arrangement is essential for breathing, as we cannot directly alter the pulmonary volume.
Respiration involves the following steps:
(i) Breathing or pulmonary ventilation by which atmospheric air is drawn in and CO2 rich alveolar air is released out.
(ii) Diffusion of gases (O2 and CO2) across alveolar membrane.
(iii) Transport of gases by the blood.
(iv) Diffusion of O2 and CO2 between blood and tissues.
(v) Utilisation of O2 by the cells for catabolic reactions and resultant release of CO2 (cellular respiration as dealt in the Chapter 14).
17.2 MECHANISM OF BREATHING
Breathing involves two stages : inspiration during which atmospheric air is drawn in and expiration by which the alveolar air is released out. The movement of air into and out of the lungs is carried out by creating a pressure gradient between the lungs and the atmosphere. Inspiration can occur if the pressure within the lungs (intra-pulmonary pressure) is less than the atmospheric pressure, i.e., there is a negative pressure in the lungs with respect to atmospheric pressure. Similarly, expiration takes place when the intra-pulmonary pressure is higher than the atmospheric pressure. The diaphragm and a specialised set of muscles - external and internal intercostals between the ribs, help in generation of such gradients. Inspiration is initiated by the contraction of diaphragm which increases the volume of thoracic chamber in the antero-posterior axis. The contraction of external inter-costal muscles lifts up the ribs and the sternum causing an increase in the volume of the thoracic chamber in the dorso-ventral axis. The overall increase in the thoracic volume causes a similar increase in pulmonary volume. An increase in pulmonary volume decreases the intra-pulmonary pressure to less than the atmospheric pressure which forces the air from outside to move into the lungs, i.e., inspiration (Figure 17.2a). Relaxation of the diaphragm and the inter-costal muscles returns the diaphragm and sternum to their normal positions and reduce the thoracic volume and thereby the pulmonary volume. This leads to an increase in intra-pulmonary pressure to slightly above the atmospheric pressure causing the expulsion of air from the lungs, i.e., expiration (Figure 17.2b). We have the ability to increase the strength of inspiration and expiration with the help of additional muscles in the abdomen. On an average, a healthy human breathes 12-16 times/minute. The volume of air involved in breathing movements can be estimated by using a spirometer which helps in clinical assessment of pulmonary functions.
17.2.1 Respiratory Volumes and Capacities
Tidal Volume (TV): Volume of air inspired or expired during a normal respiration. It is approx. 500 mL., i.e., a healthy man can inspire or expire approximately 6000 to 8000 mL of air per minute.
Inspiratory Reserve Volume (IRV): Additional volume of air, a person can inspire by a forcible inspiration. This averages 2500 mL to 3000 mL.
Expiratory Reserve Volume (ERV): Additional volume of air, a person can expire by a forcible expiration. This averages 1000 mL to 1100 mL.
Residual Volume (RV): Volume of air remaining in the lungs even after a forcible expiration. This averages 1100 mL to 1200 mL. By adding up a few respiratory volumes described above, one can derive various pulmonary capacities, which can be used in clinical diagnosis.
Inspiratory Capacity (IC): Total volume of air a person can inspire after a normal expiration. This includes tidal volume and inspiratory reserve volume ( TV+IRV).
Expiratory Capacity (EC): Total volume of air a person can expire after a normal inspiration. This includes tidal volume and expiratory reserve volume (TV+ERV).
Functional Residual Capacity (FRC): Volume of air that will remain in the lungs after a normal expiration. This includes ERV+RV.
Vital Capacity (VC): The maximum volume of air a person can breathe in after a forced expiration. This includes ERV, TV and IRV or the maximum volume of air a person can breathe out after a forced inspiration.
Total Lung Capacity (TLC): Total volume of air accommodated in the lungs at the end of a forced inspiration. This includes RV, ERV, TV and IRV or vital capacity + residual volume.
17.3 EXCHANGE OF GASES
Alveoli are the primary sites of exchange of gases. Exchange of gases also occur between blood and tissues. O2 and CO2 are exchanged in these sites by simple diffusion mainly based on pressure/concentration gradient. Solubility of the gases as well as the thickness of the membranes involved in diffusion are also some important factors that can affect the rate of diffusion. Pressure contributed by an individual gas in a mixture of gases is called partial pressure and is represented as pO2 for oxygen and pCO2 for carbon dioxide. Partial pressures of these two gases in the atmospheric air and the two sites of diffusion are given in Table 17.1 and in Figure 17.3. The data given in the table clearly indicates a concentration gradient for oxygen from alveoli to blood and blood to tissues. Similarly,
a gradient is present for CO2 in the opposite direction, i.e., from tissues to blood and blood to alveoli. As the solubility of CO2 is 20-25 times higher than that of O2, the amount of CO2 that can diffuse through the diffusion membrane per unit difference in partial pressure is much higher compared to that of O2. The diffusion membrane is made up of three major layers (Figure 17.4) namely, the thin squamous epithelium of alveoli, the endothelium of alveolar capillaries and the basement substance (composed of a thin basement membrane supporting the squamous epithelium and the basement membrane surrounding the single layer endothelial cells of capillaries) in between them. However, its total thickness is much less than a millimetre. Therefore, all the factors in our body are favourable for diffusion of O2 from alveoli to tissues and that of CO2 from tissues to alveoli.
17.4 TRANSPORT OF GASES
Blood is the medium of transport for O2 and CO2. About 97 per cent of O2 is transported by RBCs in the blood. The remaining 3 per cent of O2 is carried in a dissolved state through the plasma. Nearly 20-25 per cent of CO2 is transported by RBCs whereas 70 per cent of it is carried as bicarbonate. About 7 per cent of CO2 is carried in a dissolved state through plasma.
17.4.1 Transport of Oxygen
Haemoglobin is a red coloured iron containing pigment present in the RBCs. O2 can bind with haemoglobin in a reversible manner to form oxyhaemoglobin. Each haemoglobin molecule can carry a maximum of four molecules of O2. Binding of oxygen with haemoglobin is primarily related to partial pressure of O2. Partial pressure of CO2, hydrogen ion concentration and temperature are the other factors which can interfere with this binding. A sigmoid curve is obtained when percentage saturation of haemoglobin with O2 is plotted against the pO 2 . This curve is called the Oxygen dissociation curve (Figure 17.5) and is highly useful in studying the effect of factors like pCO2, H+ concentration, etc., on binding of O2 with haemoglobin. In the alveoli, where there is high pO2, low pCO2, lesser H+ concentration and lower temperature, the factors are all favourable for the formation of oxyhaemoglobin, whereas in the tissues, where low pO 2 , high pCO 2 , high H + concentration and higher temperature exist, the conditions are favourable for dissociation of oxygen from the oxyhaemoglobin. This clearly indicates that O 2 gets bound to haemoglobin in the lung surface and gets dissociated at the tissues. Every 100 ml of Partial pressure of oxygen (mm Hg) oxygenated blood can deliver around 5 ml of O2 to the tissues under normal physiological conditions.
17.4.2 Transport of Carbon dioxide
CO2 is carried by haemoglobin as carbamino-haemoglobin (about 20-25 per cent). This binding is related to the partial pressure of CO2. pO2 is a major factor which could affect this binding. When pCO2 is high and pO2 is low as in the tissues, more binding of carbon dioxide occurs whereas, when the pCO2 is low and pO2 is high as in the alveoli, dissociation of CO2 from carbamino-haemoglobin takes place, i.e., CO2 which is bound to haemoglobin from the tissues is delivered at the alveoli. RBCs contain a very high concentration of the enzyme, carbonic anhydrase and minute quantities of the same is present in the plasma too. This enzyme facilitates the following reaction in both directions.
$\mathrm{CO}_2 + \mathrm{H}_2\mathrm{O} \stackrel{\text{Carbonic Anhydrase}}{\underset{\longleftarrow}{\longrightarrow}}\mathrm{H}_2\mathrm{CO}_3 \stackrel{\text{Carbonic Anhydrase}}{\underset{\longleftarrow}{\longrightarrow}} \mathrm{HCO}_3^{-} + \mathrm{H}^+$
At the tissue site where partial pressure of CO2 is high due to catabolism, CO2 diffuses into blood (RBCs and plasma) and forms HCO3– and H+,. At the alveolar site where pCO2 is low, the reaction proceeds in the opposite direction leading to the formation of CO2 and H2O. Thus, CO2 trapped as bicarbonate at the tissue level and transported to the alveoli is released out as CO2 (Figure 17.4). Every 100 ml of deoxygenated blood delivers approximately 4 ml of CO2 to the alveoli.
17.5 REGULATION OF RESPIRATION
Human beings have a significant ability to maintain and moderate the respiratory rhythm to suit the demands of the body tissues. This is done by the neural system. A specialised centre present in the medulla region of the brain called respiratory rhythm centre is primarily responsible for this regulation. Another centre present in the pons region of the brain called pneumotaxic centre can moderate the functions of the respiratory rhythm centre. Neural signal from this centre can reduce the duration of inspiration and thereby alter the respiratory rate. A chemosensitive area is situated adjacent to the rhythm centre which is highly sensitive to CO2 and hydrogen ions. Increase in these substances can activate this centre, which in turn can signal the rhythm centre to make necessary adjustments in the respiratory process by which these substances can be eliminated. Receptors associated with aortic arch and carotid artery also can recognise changes in CO2 and H+ concentration and send necessary signals to the rhythm centre for remedial actions. The role of oxygen in the regulation of respiratory rhythm is quite insignificant.
17.6 DISORDERS OF RESPIRATORY SYSTEM
Asthma is a difficulty in breathing causing wheezing due to inflammation of bronchi and bronchioles.
Emphysema is a chronic disorder in which alveolar walls are damaged due to which respiratory surface is decreased. One of the major causes of this is cigarette smoking.
Occupational Respiratory Disorders: In certain industries, especially those involving grinding or stone-breaking, so much dust is produced that the defense mechanism of the body cannot fully cope with the situation. Long exposure can give rise to inflammation leading to fibrosis (proliferation of fibrous tissues) and thus causing serious lung damage. Workers in such industries should wear protective masks.
Summary
Cells utilise oxygen for metabolism and produce energy along with substances like carbon dioxide which is harmful. Animals have evolved different mechanisms for the transport of oxygen to the cells and for the removal of carbon dioxide from there. We have a well developed respiratory system comprising two lungs and associated air passages to perform this function.
The first step in respiration is breathing by which atmospheric air is taken in (inspiration) and the alveolar air is released out (expiration). Exchange of O2 and CO2 between deoxygenated blood and alveoli, transport of these gases throughout the body by blood, exchange of O2 and CO2 between the oxygenated blood and tissues and utilisation of O2 by the cells (cellular respiration) are the other steps involved.
Inspiration and expiration are carried out by creating pressure gradients between the atmosphere and the alveoli with the help of specialised muscles - intercostals and diaphragm. Volumes of air involved in these activities can be estimated with the help of spirometer and are of clinical significance.
Exchange of O2 and CO2 at the alveoli and tissues occur by diffusion. Rate of diffusion is dependent on the partial pressure gradients of O2 (pO2) and CO2 (pCO2), their solubility as well as the thickness of the diffusion surface. These factors in our body facilitate diffusion of O2 from the alveoli to the deoxygenated blood as well as from the oxygenated blood to the tissues. The factors are favourable for the diffusion of CO2 in the opposite direction, i.e., from tissues to alveoli.
Oxygen is transported mainly as oxyhaemoglobin. In the alveoli where pO2 is higher, O2 gets bound to haemoglobin which is easily dissociated at the tissues where pO2 is low and pCO2 and H+ concentration are high. Nearly 70 per cent of carbon dioxide is transported as bicarbonate (HCO3– ) with the help of the enzyme carbonic anhydrase. 20-25 per cent of carbon dioxide is carried by haemoglobin as carbamino-haemoglobin. In the tissues where pCO2 is high, it gets bound to blood whereas in the alveoli where pCO2 is low and pO2 is high, it gets removed from the blood.
Respiratory rhythm is maintained by the respiratory centre in the medulla region of brain. A pneumotaxic centre in the pons region of the brain and a chemosensitive area in the medulla can alter respiratory mechanism.
EXERCISES
1. Define vital capacity. What is its significance?
Answer Vital capacity is the maximum volume of air that can be exhaled after a maximum inspiration. It is about 3.5 - 4.5 litres in the human body. It promotes the act of supplying fresh air and getting rid of foul air, thereby increasing the gaseous exchange between the tissues and the environment.Show Answer
Answer The volume of air remaining in the lungs after a normal expiration is known as functional residual capacity (FRC). It includes expiratory reserve volume (ERV) and residual volume (RV). ERV is the maximum volume of air that can be exhaled after a normal expiration. It is about $1000 mL$ to $1500 mL$. RV is the volume of air remaining in the lungs after maximum expiration. It is about $1100 mL$ to $1500 mL$. $\therefore FRC=ERV+RV$ $\cong 1500+1500$ $\cong 3000 mL$ Functional residual capacity of the human lungs is about 2500 - $3000 mL$.Show Answer
Answer Each alveolus is made up of highly-permeable and thin layers of squamous epithelial cells. Similarly, the blood capillaries have layers of squamous epithelial cells. Oxygen-rich air enters the body through the nose and reaches the alveoli. The deoxygenated (carbon dioxide-rich) blood from the body is brought to the heart by the veins. The heart pumps it to the lungs for oxygenation. The exchange of $O_2$ and $CO_2$ takes place between the blood capillaries surrounding the alveoli and the gases present in the alveoli. Thus, the alveoli are the sites for gaseous exchange. The exchange of gases takes place by simple diffusion because of pressure or concentration differences. The barrier between the alveoli and the capillaries is thin and the diffusion of gases takes place from higher partial pressure to lower partial pressure. The venous blood that reaches the alveoli has lower partial pressure of $O_2$ and higher partial pressure of $CO_2$ as compared to alveolar air. Hence, oxygen diffuses into blood. Simultaneously, carbon dioxide diffuses out of blood and into the alveoli.Show Answer
Answer Plasma and red blood cells transport carbon dioxide. This is because they are readily soluble in water. (1) Through plasma: About $7 %$ of $CO_2$ is carried in a dissolved state through plasma. Carbon dioxide combines with water and forms carbonic acid. $
CO_2+H_2 O \longrightarrow \underset{\text{(Carbonic acid)}}{H_2 CO_3}
$ Since the process of forming carbonic acid is slow, only a small amount of carbon dioxide is carried this way. (2) Through RBCs: About 20 â€" $25 %$ of $CO_2$ is transported by the red blood cells as carbaminohaemoglobin. Carbon dioxide binds to the amino groups on the polypeptide chains of haemoglobin and forms a compound known as carbaminohaemoglobin. (3) Through sodium bicarbonate: About $70 %$ of carbon dioxide is transported as sodium bicarbonate. $As^{2} CO_2$ diffuses into the blood plasma, a large part of it combines with water to form carbonic acid in the presence of the enzyme carbonic anhydrase. Carbonic anhydrase is a zinc enzyme that speeds up the formation of carbonic acid. This carbonic acid dissociates into bicarbonate $(HCO_3{ }^{-})$ and hydrogen ions $(H^{+})$. $
CO_2+H_2 O \xrightarrow{\text{ Carbonic anhydrase }} H_2 CO_3
$ $
\mathrm{H_2CO_3} \xrightarrow[\text{Carbonic}]{\text{anhydrase}} + \mathrm{HCO_3^-} + \mathrm{H^+}
$Show Answer
(i) $pO_2$ lesser, $pCO_2$ higher
(ii) $pO_2$ higher, $pCO_2$ lesser
(iii) $pO_2$ higher, $pCO_2$ higher
(iv) $pO_2$ lesser, $pCO_2$ lesser
Answer (ii) $pO_2$ higher, $pCO_2$ lesser The partial pressure of oxygen in atmospheric air is higher than that of oxygen in alveolar air. In atmospheric air, $pO_2$ is about $159 mm Hg$. In alveolar air, it is about $104 mm Hg$. The partial pressure of carbon dioxide in atmospheric air is lesser than that of carbon dioxide in alveolar air. In atmospheric air, $pCO_2$ is about $0.3 mmHg$. In alveolar air, it is about $40 mm Hg$.Show Answer
Answer Diaphragm contracts Inspiration or inhalation is the process of bringing air from outside the body into the lungs. It is carried out by creating a pressure gradient between the lungs and the atmosphere. When air enters the lungs, the diaphragm contracts toward the abdominal cavity, thereby increasing the space in the thoracic cavity for accommodating the inhaled air. The volume of the thoracic chamber in the anteroposterior axis increases with the simultaneous contraction of the external intercostal muscles. This causes the ribs and the sternum to move out, thereby increasing the volume of the thoracic chamber in the dorsoventral axis. The overall increase in the thoracic volume leads to a similar increase in the pulmonary volume. Now, as a result of this increase, the intra-pulmonary pressure becomes lesser than the atmospheric pressure. This causes the air from outside the body to move into the lungs.Show Answer
Answer The respiratory rhythm centre present in the medulla region of the brain is primarily responsible for the regulation of respiration. The pneumotaxic centre can alter the function performed by the respiratory rhythm centre by signalling to reduce the inspiration rate. The chemosensitive region present near the respiratory centre is sensitive to carbon dioxide and hydrogen ions. This region then signals to change the rate of expiration for eliminating the compounds. The receptors present in the carotid artery and aorta detect the levels of carbon dioxide and hydrogen ions in blood. As the level of carbon dioxide increases, the respiratory centre sends nerve impulses for the necessary changes.Show Answer
Answer $pCO_2$ plays an important role in the transportation of oxygen. At the alveolus, the low $pCO_2$ and high $pO_2$ favours the formation of haemoglobin. At the tissues, the high $pCO_2$ and low $pO_2$ favours the dissociation of oxygen from oxyhaemoglobin. Hence, the affinity of haemoglobin for oxygen is enhanced by the decrease of $pCO_2$ in blood. Therefore, oxygen is transported in blood as oxyhaemoglobin and oxygen dissociates from it at the tissues.Show Answer
Answer As altitude increases, the oxygen level in the atmosphere decreases. Therefore, as a man goes uphill, he gets less oxygen with each breath. This causes the amount of oxygen in the blood to decline. The respiratory rate increases in response to the decrease in the oxygen content of blood. Simultaneously, the rate of heart beat increases to increase the supply of oxygen to blood.Show Answer
Answer In insects, gaseous exchange occurs through a network of tubes collectively known as the tracheal system. The small openings on the sides of an insect’s body are known as spiracles. Oxygen-rich air enters through the spiracles. The spiracles are connected to the network of tubes. From the spiracles, oxygen enters the tracheae. From here, oxygen diffuses into the cells of the body. The movement of carbon dioxide follows the reverse path. The $CO_2$ from the cells of the body first enters the tracheae and then leaves the body through the spiracles.Show Answer
Answer The oxygen dissociation curve is a graph showing the percentage saturation of oxyhaemoglobin at various partial pressures of oxygen. The curve shows the equilibrium of oxyhaemoglobin and haemoglobin at various partial pressures. In the lungs, the partial pressure of oxygen is high. Hence, haemoglobin binds to oxygen and forms oxyhaemoglobin. Tissues have a low oxygen concentration. Therefore, at the tissues, oxyhaemoglobin releases oxygen to form haemoglobin. The sigmoid shape of the dissociation curve is because of the binding of oxygen to haemoglobin. As the first oxygen molecule binds to haemoglobin, it increases the affinity for the second molecule of oxygen to bind. Subsequently, haemoglobin attracts more oxygen.Show Answer
Answer Hypoxia is a condition characterised by an inadequate or decreased supply of oxygen to the lungs. It is caused by several extrinsic factors such as reduction in $pO_2$, inadequate oxygen, etc. The different types of hypoxia are discussed below. Hypoxemic hypoxia In this condition, there is a reduction in the oxygen content of blood as a result of the low partial pressure of oxygen in the arterial blood. Anaemic hypoxia In this condition, there is a reduction in the concentration of haemoglobin. Stagnant or ischemic hypoxia In this condition, there is a deficiency in the oxygen content of blood because of poor blood circulation. It occurs when a person is exposed to cold temperature for a prolonged period of time. Histotoxic hypoxia In this condition, tissues are unable to use oxygen. This occurs during carbon monoxide or cyanide poisoning.Show Answer
(a) IRV and ERV
(b) Inspiratory capacity and Expiratory capacity.
(c) Vital capacity and Total lung capacity.
Answer (a) (b) (c)Show Answer
Inspiratory reserve volume (IRV)
Expiratory reserve volume (ERV)
1. It is the maximum volume of air that can be inhaled after a normal inspiration.
1. It is the maximum volume of air that can be exhaled after a normal expiration.
2. It is about 2500 to 3500 mL in the human lungs.
2. It is about 1000 to 1100 mL in the human lungs.
Inspiratory capacity (IC)
Expiratory capacity(EC)
1. It is the volume of air that can be inhaled after a normal expiration.
1. It is the volume of air that can be exhaled after a normal inspiration.
2. It includes tidal volume and inspiratory reserve volume.
2. It includes tidal volume and expiratory reserve volume.
$IC=TV+IRV$
$EC=TV+ERV$
Vital capacity (VC)
Total lung capacity (TLC)
1. It is the maximum volume of air that can be exhaled after a maximum inspiration. It includes IC and ERV.
1. It is the volume of air in the lungs after maximum inspiration. It includes IC, ERV, and residual volume.
2. It is about 4000 mL in the human lungs.
2. It is about 5000 to 6000 mL in the human lungs.
Show Answer
Answer
Tidal volume is the volume of air inspired or expired during normal respiration.
It is about 6000 to $8000 mL$ of air per minute.
The hourly tidal volume for a healthy human can be calculated as:
Tidal volume $=6000$ to $8000 mL /$ minute
Tidal volume in an hour $=6000$ to $8000 mL \times(60 min)$ $=3.6 \times 10^{5} mL$ to $4.8 \times 10^{5} mL$
Therefore, the hourly tidal volume for a healthy human is approximately $3.6 \times 10^{5} mL$ to $4.8 \times 10^{5} mL$.
