Optics 5 Question 1

1. The value of numerical aperture of the objective lens of a microscope is 1.25 . If light of wavelength 5000\AA is used, the minimum separation between two points, to be seen as distinct, will be

(2019 Main, 12 April I)

(a) 0.24μm

(b) 0.38μm

(c) 0.12μm

(d) 0.48μm

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Answer:

Correct Answer: 1. (a)

Solution:

  1. Key Idea Numerical Aperture (NA) of the microscope is given by NA=0.61λd

Here, d= minimum separation between two points to be seen as distinct and λ =wavelength of light.

Given, λ=5000\AA=5000×1010m and

NA=1.25

Now, d=0.61λNA=0.61×5000×10101.25

or

d=3.051.25×107m=2.4×107md=0.24μm

or

2 Limit of resolution for a telescope from Rayleigh’s criteria is

θR=1.22λD

Here, D=250cm=250×102m

and λ=600nm=600×109m

So, limit of resolution is

θR=1.22×600×109250×102=2.93×107rad3×107rad

3 For a telescope, limit of resolution is

 given by Δθ=1.22λD

Here, λ=500nm=500×109m,

D=200cm=200×102m

So, limit of resolution is Δθ=1.22×500×109200×102

=305×109rad



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