<h3 id="successprotein-structure-function-relationshipsuccess"><Success>Protein structure function relationship</Success></h3> <div style='float: left; width:50%;'> </div> <div style='float: right; width:0%;'> <p><img alt="image" src="https://temp-public-img-folder.s3.ap-south-1.amazonaws.com/sathee.prutor.images/subject-images/iitpal/image/biology-class-12-unit-16-chapter-03-protein-structure-function-relationship-lecture-3_6-9st8hatjlaw-026-0034.8.jpg"></p> </div>

<h3 id="successprotein-foldingsuccess"><Success>Protein folding</Success></h3> <div style='float: left; width:50%;'> </div> <div style='float: right; width:100%;'> <!----> <img src="https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/b2c1495b-a71a-42f1-e102-e2aabee6d800/public" width="70%"> </div>

<h3 id="successproteins-with-different-sequences-may-give-similar-foldssuccess"><Success>Proteins with different sequences may give similar folds</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <p>α-lactalbumin + Galactosyltransferase Galactose $\rarr$ Glucose=ẞ 1-4 linkage, Lactose</p> <p>Lysozyme-hydrolysis of a Beta-1,4 glycosidic linkage in polysaccharides</p> </div> <div style='float: right; width:50%;'> <!----> <img src= "https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/a107d838-edeb-49d2-0e82-331610f12500/public" style='width:60%'> </div>

<h3 id="successproteinaceous-food-processing-enzyme-proteasessuccess"><Success>Proteinaceous Food processing enzyme (Proteases)</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <ul> <li>Trypsin and Chymotrypsin</li> </ul> </div> <div style='float: right; width:100%;'> <!----> <img src="https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/84189097-32ff-4578-edef-1581c127c900/public" width="60%"> </div>

<h3 id="successproteinaceous-food-processing-enzyme-proteasessuccess-1"><Success>Proteinaceous food processing enzyme (Proteases)</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <ul> <li>The enzyme chymotrypsin is made up of a linear chain of 245 amino acids interrupted into three peptides-A,B,C</li> <li>The protein folds into a globular structure. In the 3-D structure of the enzyme three amino acid his57, asp102 and ser195 come close together in space which allows a “charge relay system to operate</li> </ul> </div> <div style='float: right; width:50%;'> <!----> <img src= "https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/f3e5a1f4-7590-426f-b52b-6996e8eb5d00/public" style='width:100%'> </div>

<div> <h3 id="successproteinaceous-food-processing-enzyme-proteasessuccess-2"><Success>Proteinaceous food processing enzyme (Proteases)</Success></h3> </div> <div style='float: left; width:100%;font-size:30px;text-align:left;'> <ol> <li>Normally the hydroxyl group of a serine residue is not acidic</li> <li>In chymotrypsin; only ser 195 becomes acidic due to the unique constellation of the three amino acid residues because the protein has folded uniquely in space</li> <li>The specific site of chymotrypsin is a large space created within the enzyme active site and lined by hydrophobic residues which only allow bulky aromatic, hydrophobic amino acids to bind</li> </ol> </div>

<h3 id="successchymotrypsin-catalysissuccess"><Success>Chymotrypsin catalysis</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <ol> <li>Normally the hydroxyl group of a serine residue is not acidic</li> <li>In chymotrypsin; only ser 195 becomes acidic due to the unique constellation of the three amino acid residues because the protein has folded uniquely in space</li> </ol> </div> <div style='float: right; width:50%;'> <!----> <img src= "https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/4efa3ac2-4b73-48ec-0e03-f9081e054d00/public" style='width:100%'> </div>

<div> <h3 id="successchymotrypsin-catalysissuccess-1"><Success>Chymotrypsin catalysis</Success></h3> </div> <div style='float: left; width:100%;font-size:30px;text-align:left;'> <ol start="3"> <li>The specific site of chymotrypsin is a large space created within the enzyme active site and lined by hydrophobic residues which only allow bulky aromatic, hydrophobic amino acids to bind</li> </ol> </div>

<h3 id="successother-enzyme-follow-this-tricksuccess"><Success>Other enzyme follow this trick</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <ul> <li>Nature repeats this in a variety of other enzymes</li> <li>Trypsin, subtilisin, thrombin and the brain enzyme, acetyl choline esterase</li> <li>All have a reactive serine residue which is central to the catalytic mechanism</li> </ul> </div> <div style='float: right; width:50%;'> <!----> <img src="https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/7c6f0b19-cce2-47c4-4b22-0f37d0a22c00/public" width="100%"> </div>

<div> <h3 id="successother-enzyme-follow-this-tricksuccess-1"><Success>Other enzyme follow this trick</Success></h3> </div> <div style='float: left; width:100%;font-size:30px;text-align:left;'> <ul> <li>Acetylcholine esterase inhibitors (serine binding)</li> <li>Nowadays derivatives of organophosphates such as malathion and parathion which are not toxic to humans are used as mosquito repellants (Mortein, Good Knight) by effecting nerve transmission in insects</li> </ul> </div>

<h3 id="successmolecular-disease--sickle-cell-anemiasuccess"><Success>Molecular disease- sickle cell anemia</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> <ul> <li>It is caused by a change of amino acids in the 6th position of β globin (Glu to Val)</li> <li>The mutation results in: 1) arrays of aggregates of hemoglobin molecules, 2) deformation of the red blood cell, and 3) clotting in blood vessels and tissues</li> </ul> </div> <div style='float: right; width:50%;'> <!----> <img src="https://imagedelivery.net/YfdZ0yYuJi8R0IouXWrMsA/3651f898-3faf-43f0-29eb-d90480d39400/public" width="100%"> </div>

<h3 id="successthank-yousuccess"><Success>Thank you</Success></h3> <div style='float: left; width:50%; font-size:30px; text-align:left;'> </div> <div style='float: right; width:0%;'> <p><img alt="image" src="https://temp-public-img-folder.s3.ap-south-1.amazonaws.com/sathee.prutor.images/subject-images/iitpal/image/biology-class-12-unit-16-chapter-03-protein-structure-function-relationship-lecture-3_6-9st8hatjlaw-262-2128.8.jpg"></p> </div>