Curd formation
$$ C_6 ~ H_{12} ~ O_6 \text {(Glucose)} + 2 ADP + 2 P_i$$
$$ \hspace{1cm}\downarrow $$
$$2C_2 ~ H_5 ~ OH \text {(Ethanol)} + 2C O_2 + 2 ATP$$
Bread is made by fermenting dough with baker’s yeast
TODDY-A traditional drink of south India is obtained by fermenting sap from palms
Food items prepared by fermenting fishes, soyabe answer and bamboo shoots
Swiss cheese has large holes due to production of large amount of $CO_2$ by propionibacterium sharmanii
Roquefort cheese are ripened by growing a specific fungi which gives it a particular flavour
Yeast have been used from time immemorial for production of beverages
Obtained by fermentation of malted cereals and fruit juices
Wine and bear are produced without distillation
Whisky Brandy and rum are produced by distillation of fermented broth
Anti $\rightarrow$ against and Bio $\rightarrow$ life, together meaning" Against life $\rightarrow$ in context of disease causing organisms
Antibiotics are chemical substances which are produced by some microbes and can kill or retard the growth of other microbes
Penicillin was first antibiotic to be discovered by Alexander Flemming while working on Staphylococci bacteria
Full potential of Penicillin as an effective antibiotic was established by Ernest Chain and Howard Florey
It was extensively used to treat soldiers in World War
Flemming Ernest Cham and Howard Florey also received Nobel prize in 1945 for this discovery
Microbe | Acid produced |
---|---|
Aspergillus niger | Citric Acid |
Acetobacter aceti | Acetic acid |
Clostridium butylicum | Butyric Acid |
Lactobacillus | Lactic acid |
Bioactive molecules
Cyclosporin A which is obtained from the fungus ‘Trichoderma polvsporum’ is used as an immunosuppresive agent
Statins produced by the yeast ‘Monascus purpureus’ is used as blood cholesterol lowering agent
Various enzymes produced by microbes -
Lipases $\rarr$ used in detergents
Pectinases and Proteases → clarifying bottled fruit juices
Streptokinase $\rarr$ used as ‘clot buster’
Environmental issues are of utmost concern to us today
Rapidly increasing pollution is one of our biggest problem
Can microbes help us in solving these problems?
Bioremediation refers to use of organisms in cleaning of pollution
It involves physical removal of large and small particles
Floating debis are first removed through screens by sequential filtration
Grit (sand and pebbles) are removed by sedimentation
Solid that settle forms primary sludge and the supernatant forms Effluent
Primary effluent is passed into large aeration tanks
Mechanical agitation and pumping of air allows vigorous growth of useful microbes into flocs
Flocs are masses of bacteria associated with fungal filaments to form mesh like structures
Majority of organic matter in the effluent is consumed by these microbes
This reduces the Biological oxygen demand (Answerwer)
BOD refers to the amount of oxygen that would be consumed if all the organic matter in one litre of water were oxidised by bacteria
Greater the BOD, more is the pollution in water
Once there is a significant reduction in BOD, the bacterial flock are allowed to sediment
This sediment is called “Activated sludge which can again be used as inoculum (a small part) when pumped back into the aeration tank
Remaining major part is pumped into large tanks called anaerobic sludge digestors
The anaerobic bacteria present in it further digest the bacteria and fungi in the sludge producing a mixture of gases known as “Biogas”
The effluent is further treated with disinfectants before releasing into rivers and streams
Ganga’s sorrow
Nearly 40,000 long km drains not cleaned for over 50 years falling into the river at different points. They also carry urban and chemical discharge
Varanasi alone discharges 200 million litres of untreated human sewage into the river causing Faecal Coliform Bacteria (FCB). Official standards state 500 ml faecal coliforms per 100 ml is safe for bathing in Varanasi it is 120 times high 60,000 FCB
Today we learnt how most of the microbes can actually be beneficial for us
We learnt about the applications of microbes, their processes and products in
Household products
Industrial products
Bioremediation
Question 1. Bacteria can not be seen with the naked eyes, but these can be seen with the help of a microscope. If you have to carry a sample, which sample would you take and why?
Answer. Curd contains Lactobacillus and is most common household product
Question 2. Give examples to prove that microbes release gases during metabolism
Puffed up appearance of bread, dosa, idli etc
Large holes in Swiss Cheese
Question 3. In which food would you find lactic acid bacteria? Mention some of their uses
Answer. In curd, Lactobacillus convert lactose into lactic acid. Improves its nutritional value by increasing Vitamin $ B_{12} $
Question 4. Name some traditional Indian foods made of wheat, rice and Bengal gram which involve use of microbes
Answer. Dosa and Idli
Question 5. In which ways have microbes played a major role in controlling diseases caused by harmful bacteria
Answer. Antibiotics produced by microbes help in controlling the growth of other disease causing microbes
Question 6. Name any two species of fungus which are used in the production of antibiotics
Answer. Penicillin notatum, Aspergillus fumigatus, Clavicep purpurea
Question 7. What is sewage? In which way can sewage be harmful to us?
Answer. All the domestic and industrial wastewater is the sewage. Its harmful effects are
• Spread of water borne diseases
• Reduced Disolved oxygen content may kill aquatic life
Question 8. What is the key difference between primary and secondary sewage treatments!
Answer. Primary treatment is physical or mechanical while Secondary treatment is biological due to microbial activity
Question 1. Critically analyze the role of microrganisms in sustaining of our ecosystem
Question 2. What according to you is the most distinguishing feature of microorganisms that enables them to be used for commercial production?
Question 3. Find out from magazines or newspapers about any latest research on beneficial aspects of microbes
Question 4. Can you suggest some examples in nature where microbes have proved to be beneficial
Questoin 5. Some of the archaeabacteria are extremophiles. How are they able to survive in such extreme conditions?