Biotechnology Principles And Processes Dna
Biotechnology- Principles and Processes - DNA
DNA
: Deoxyribose Nucleic Acid
Consists of two strands of nucleotides
Complementary base pairing (A-T, C-G)
Double helix structure
Biotechnology Principles And Processes Dna
Structure of DNA
Sugar-phosphate backbone
Nitrogenous bases
: Adenine (A), Thymine (T), Cytosine (C), Guanine (G)
Base pairing rules
: A-T, C-G
Antiparallel strands
Biotechnology Principles And Processes Dna
DNA Replication
Semiconservative replication
Replication fork formation
Enzymes involved
: DNA Helicase, DNA Polymerase, DNA Ligase
Leading and lagging strands
Replication results in two identical DNA molecules
Biotechnology Principles And Processes Dna
Genetic Engineering
Recombinant DNA technology
Manipulation of DNA sequences
Tools used
: Restriction enzymes, DNA ligase, PCR
Applications
: Production of medicines, genetic modification of crops
Biotechnology Principles And Processes Dna
Restriction Enzymes
Enzymes that cut DNA at specific recognition sites
Produce sticky or blunt ends
Used in genetic engineering to create recombinant DNA
Biotechnology Principles And Processes Dna
DNA Cloning
Production of identical copies of DNA
Steps
: Isolation of target gene, Insertion into cloning vector, Transformation, Selection and identification of clones
Biotechnology Principles And Processes Dna
PCR Polymerase Chain Reaction
Technique for amplifying DNA
Steps
: Denaturation, Annealing, Extension
Uses
: Diagnosis of genetic diseases, DNA fingerprinting, Gene cloning
Biotechnology Principles And Processes Dna
Gel Electrophoresis
Technique used to separate DNA fragments
Principle
: DNA fragments move towards positive electrode based on size
Analysis of DNA profiles, paternity testing, forensic investigations
Biotechnology Principles And Processes Dna
DNA Sequencing
Determination of nucleotide sequence in a DNA molecule
Methods
: Sanger sequencing, Next-generation sequencing
Applications
: Genome mapping, gene sequencing
Biotechnology Principles And Processes Dna
Gene Therapy
Introduction to gene therapy
Gene therapy methods
: Insertion of functional genes, Repair of mutated genes, Deactivation of harmful genes
Applications of gene therapy
: Treatment of genetic disorders, Cancer therapy, Vaccines
Biotechnology Principles And Processes Dna
CRISPR-Cas9
CRISPR-Cas9
: Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated protein 9
Gene editing tool
Mechanism of action
: Recognition of target DNA sequence, Cleavage of DNA, DNA repair process
Applications
: Disease treatment, Crop improvement, Creation of animal models
Biotechnology Principles And Processes Dna
GMOs Genetically Modified Organisms
Definition and types of GMOs
Genetic modification techniques
: Transgenic organisms, Gene knockout, Gene stacking
Applications of GMOs
: Improved crop yield, Pest resistance, Disease resistance
Biotechnology Principles And Processes Dna
Stem Cells
Introduction to stem cells
Types of stem cells
: Embryonic stem cells, Adult stem cells, Induced pluripotent stem cells
Differentiation potential of stem cells
Applications of stem cells
: Tissue regeneration, Disease treatment, Drug discovery
Biotechnology Principles And Processes Dna
Cloning Techniques
Different types of cloning
: Reproductive cloning, Therapeutic cloning
Process of reproductive cloning
Process of therapeutic cloning
Ethical concerns and controversies surrounding cloning
Biotechnology Principles And Processes Dna
DNA Fingerprinting
Principle and applications of DNA fingerprinting
Steps involved in DNA fingerprinting
: DNA extraction, Amplification of DNA, Gel electrophoresis, DNA analysis
Uses of DNA fingerprinting
: Forensic investigations, Paternity testing, Identification of species
Biotechnology Principles And Processes Dna
Bioremediation
Definition of bioremediation
Types of bioremediation
: Microbial bioremediation, Phytoremediation
Role of microorganisms in bioremediation
Applications of bioremediation
: Clean-up of oil spills, Removal of toxic chemicals
Biotechnology Principles And Processes Dna
Synthetic Biology
Introduction to synthetic biology
Combination of biology and engineering
Design and construction of new biological parts and systems
Applications of synthetic biology
: Designing new drugs, Creating sustainable energy sources
Biotechnology Principles And Processes Dna
Genetic Counseling
Definition and purpose of genetic counseling
Process of genetic counseling
: Evaluation of family and medical history, Genetic testing, Communication of risk and options
Benefits and ethical considerations of genetic counseling
Biotechnology Principles And Processes Dna
Human Genome Project
Overview of the Human Genome Project
Aims and objectives of the project
Methods used for sequencing the human genome
Significance of the project in understanding diseases and human diversity
Biotechnology Principles And Processes Dna
Recombinant DNA Technology
Introduction to recombinant DNA technology
Techniques involved
: Cloning, DNA sequencing, Gene expression
Applications
: Production of therapeutic proteins, Creation of transgenic organisms, Gene therapy
Biotechnology Principles And Processes Dna
Gene Expression
Definition of gene expression
Transcription
: Synthesis of mRNA from DNA template
Translation
: Synthesis of protein from mRNA template
Regulation of gene expression
: Transcription factors, Promoters, Enhancers, Repressors
Biotechnology Principles And Processes Dna
Transcription
Initiation
: Binding of RNA polymerase to promoter region
Elongation
: Addition of nucleotides to growing RNA strand
Termination
: Release of RNA molecule from DNA template
Role of transcription factors and regulatory elements
Biotechnology Principles And Processes Dna
Translation
Ribosome structure and function
Initiation
: Assembly of ribosome at start codon
Elongation
: Stepwise addition of amino acids to growing polypeptide chain
Termination
: Release of polypeptide at stop codon
Biotechnology Principles And Processes Dna
Genetic Code
Definition of genetic code
Codons
: Three-nucleotide sequences that code for specific amino acids
Start codon (AUG) and stop codons (UAA, UAG, UGA)
Degeneracy and universality of the genetic code
Biotechnology Principles And Processes Dna
Mutations
Types of mutations
: Point mutations, Insertions, Deletions, Frameshift mutations
Causes of mutations
: Spontaneous, Induced by mutagens
Effects of mutations
: Silent mutations, Missense mutations, Nonsense mutations
Biotechnology Principles And Processes Dna
Operons
Definition of operons
Structure and components of operons
: Promoter, Operator, Structural genes
Inducible and repressible operons
Examples
: Lac operon, Trp operon
Biotechnology Principles And Processes Dna
Genetic Disorders
Introduction to genetic disorders
Types of genetic disorders
: Single gene disorders, Chromosomal disorders, Multifactorial disorders
Examples of genetic disorders
: Cystic fibrosis, Down syndrome, Diabetes
Biotechnology Principles And Processes Dna
Evolutionary Biology
Introduction to evolutionary biology
Mechanisms of evolution
: Natural selection, Genetic drift, Gene flow, Mutation
Evidence for evolution
: Fossil record, Homologous structures, DNA sequence comparisons
Speciation and evolutionary patterns
Biotechnology Principles And Processes Dna
Human Evolution
Overview of human evolution
Hominid evolution
: Australopithecus, Homo habilis, Homo erectus, Homo sapiens
Cultural and technological advancements in human evolution
Impact of human evolution on societies and genetic diversity