Biomolecules Amino Acids
Biomolecules - Amino Acids
Amino acids are organic compounds that contain both an amino group (-NH2) and a carboxyl group (-COOH)
They are building blocks of proteins
Amino acids are characterized by a central carbon atom called alpha carbon (α-carbon)
There are 20 different amino acids commonly found in proteins
Each amino acid is unique due to the side chain, also known as R-group
Biomolecules Amino Acids
Classification of Amino Acids
Non-Polar Amino Acids
Side chains are hydrophobic
Examples: Glycine, Alanine, Valine
Polar Amino Acids
Side chains are hydrophilic
Examples: Serine, Threonine, Glutamine
Acidic Amino Acids
Side chains are negatively charged at physiological pH
Examples: Aspartic acid, Glutamic acid
Basic Amino Acids
Side chains are positively charged at physiological pH
Examples: Lysine, Arginine, Histidine
Biomolecules Amino Acids
Structure of Amino Acids
Amino acids have a common structure consisting of an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom (H), and an R-group
The amino group and the carboxyl group are attached to the α-carbon
The R-group varies between different amino acids, giving them distinct properties
Biomolecules Amino Acids
Essential Amino Acids
Essential amino acids cannot be synthesized by the human body and must be obtained from the diet
There are 9 essential amino acids
: Phenylalanine, Valine, Threonine, Tryptophan, Isoleucine, Methionine, Leucine, Lysine, and Histidine
Insufficient intake of essential amino acids can lead to protein malnutrition
Biomolecules Amino Acids
Peptide Bond Formation
Amino acids are joined together through a peptide bond
The reaction between the carboxyl group of one amino acid and the amino group of another amino acid forms a peptide bond
The resulting molecule is called a dipeptide
Water is released during the formation of peptide bond (condensation reaction)
Biomolecules Amino Acids
Primary Structure of Proteins
The primary structure of a protein is the linear sequence of amino acids
It is determined by the order and number of amino acids in the polypeptide chain
A change in just one amino acid can significantly affect the protein’s function
Biomolecules Amino Acids
Secondary Structure of Proteins
Secondary structure refers to the local folding patterns within a protein
Two common secondary structures are alpha helix and beta sheet
Alpha helix is a right-handed coiled structure stabilized by hydrogen bonding between amino acid residues
Beta sheet consists of multiple strands of extended polypeptide chains held together by hydrogen bonds
Biomolecules Amino Acids
Tertiary Structure of Proteins
Tertiary structure refers to the overall three-dimensional folding of a protein
It is stabilized by various interactions such as hydrogen bonds, disulfide bridges, ionic interactions, and hydrophobic interactions
Tertiary structure determines the protein’s overall shape and function
Biomolecules Amino Acids
Quaternary Structure of Proteins
Quaternary structure refers to the arrangement of multiple polypeptide chains (subunits) in a protein complex
Protein subunits can be identical or different
Quaternary structure is stabilized by the same types of interactions as tertiary structure
Biomolecules Amino Acids
Protein Denaturation
Denaturation is the disruption of a protein’s structure, resulting in loss of its biological activity
Denaturation can be caused by heat, pH extremes, exposure to certain chemicals, or mechanical agitation
Denatured proteins usually lose their secondary, tertiary, and quaternary structures
Biomolecules Amino Acids
Protein Folding
Protein folding is the process by which a protein adopts its functional three-dimensional structure
It occurs spontaneously, guided by the protein’s amino acid sequence and various interactions
Misfolding of proteins can lead to diseases such as Alzheimer’s and Parkinson’s
Biomolecules Amino Acids
Protein Functions
Proteins have a wide range of functions in living organisms
:
Enzymes: Catalyze biochemical reactions
Structural Proteins: Provide support and framework to cells and tissues
Transport Proteins: Carry molecules across cell membranes
Hormones: Regulate various physiological processes
Antibodies: Defend against pathogens
Receptors: Receive and transmit signals in cells
Biomolecules Amino Acids
Carbohydrates
Carbohydrates are organic compounds composed of carbon, hydrogen, and oxygen in a ratio of 1:2
:2:1
Main functions include energy storage and structural support
Monosaccharides are the simplest carbohydrates (e.g., glucose, fructose)
Disaccharides are formed by the condensation of two monosaccharides (e.g., sucrose, lactose)
Polysaccharides are complex carbohydrates formed by the polymerization of monosaccharides (e.g., starch, cellulose)
Biomolecules Amino Acids
Lipids
Lipids are hydrophobic molecules that include fats, oils, waxes, and steroids
Functions of lipids include long-term energy storage, insulation, and protection of organs
Fatty acids are the building blocks of lipids
Triglycerides are formed from glycerol and three fatty acid molecules
Phospholipids have a hydrophilic head and hydrophobic tails, making them essential for cell membranes
Biomolecules Amino Acids
Nucleic Acids
Nucleic acids are macromolecules that store and transmit genetic information
Two types of nucleic acids
: DNA (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid)
DNA carries the genetic code and is responsible for inherited traits
RNA plays a role in protein synthesis and gene expression
Nucleotides are the building blocks of nucleic acids, consisting of a phosphate group, a sugar (ribose or deoxyribose), and a nitrogenous base (adenine, guanine, cytosine, thymine/uracil)
Biomolecules Amino Acids
DNA Structure
DNA is a double-stranded helix composed of two antiparallel strands held together by hydrogen bonds
The complementary base pairing rule
: Adenine (A) with Thymine (T) and Guanine (G) with Cytosine (C)
The structure of DNA was determined by Watson and Crick in 1953
The discovery of the DNA structure is one of the most significant findings in the history of biology
Biomolecules Amino Acids
DNA Replication
DNA replication is the process by which DNA is duplicated to produce identical copies
It occurs during the S phase of the cell cycle
The double helix is unwound, and each strand serves as a template for the synthesis of a new complementary strand
DNA replication is a highly accurate process, with an error rate of approximately 1 in 10 billion nucleotides
Biomolecules Amino Acids
RNA Structure
RNA is a single-stranded nucleic acid that plays various roles in protein synthesis and gene regulation
It contains ribose sugar instead of deoxyribose and the base uracil (U) instead of thymine (T)
Types of RNA include messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA)
RNA molecules can fold into complex structures, allowing them to perform diverse functions
Biomolecules Amino Acids
Transcription
Transcription is the process by which DNA is “copied” into RNA
It occurs in the nucleus of eukaryotic cells
RNA polymerase binds to the promoter region of a gene, unwinds the DNA double helix, and synthesizes an RNA molecule using one strand of DNA as a template
The resulting RNA molecule is called the primary transcript or pre-mRNA
Biomolecules Amino Acids
Translation
Translation is the process by which an mRNA molecule is translated into a sequence of amino acids to produce a protein
It occurs in the cytoplasm on ribosomes
Transfer RNA (tRNA) molecules bring amino acids to the ribosome, matching their anticodon to the mRNA codon
The ribosome catalyzes the formation of peptide bonds between the amino acids, resulting in the synthesis of a polypeptide chain
Biomolecules Amino Acids
Protein Synthesis
Protein synthesis is the process by which cells build proteins based on the instructions encoded in DNA
It involves two main steps
: transcription and translation
Transcription takes place in the nucleus, where DNA is transcribed into mRNA
Translation occurs in the cytoplasm, where mRNA is translated into a sequence of amino acids to form a protein
Biomolecules Amino Acids
Protein Structure Determination
Determining the structure of proteins is crucial for understanding their functions and roles in biological processes
X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy are commonly used methods for protein structure determination
X-ray crystallography involves the analysis of diffraction patterns produced by X-rays passing through a crystalline protein sample
NMR spectroscopy uses the interaction of a protein with magnetic fields to study its structure
Biomolecules Amino Acids
Enzymes
Enzymes are biological catalysts that accelerate the rate of chemical reactions in living organisms
They are typically proteins, but certain RNA molecules called ribozymes can also act as enzymes
Enzymes lower the activation energy of reactions, allowing them to occur more rapidly
Enzyme activity is influenced by factors such as temperature, pH, and substrate concentration
Biomolecules Amino Acids
Enzyme Kinetics
Enzyme kinetics is the study of how enzymes function and their reaction rates
The Michaelis-Menten equation describes the relationship between enzyme activity and substrate concentration
Enzymes exhibit saturation kinetics, meaning that as substrate concentration increases, the reaction rate levels off at the enzyme’s maximum velocity (Vmax)
The substrate concentration at which the reaction rate is half of Vmax is called the Michaelis constant (Km)
Biomolecules Amino Acids
Reaction Mechanisms
Enzymes catalyze reactions by following specific reaction mechanisms
The lock-and-key model suggests that enzymes have an active site with a specific shape that matches the substrate’s shape
The induced-fit model proposes that the enzyme’s active site undergoes conformational changes upon substrate binding
Enzymes can perform various types of reactions, including oxidation-reduction, hydrolysis, and isomerization
Biomolecules Amino Acids
Metabolism
Metabolism refers to the sum of all chemical reactions that occur in an organism
Anabolism involves the synthesis of complex molecules from simpler ones, typically requiring energy
Catabolism involves the breakdown of complex molecules into simpler ones, releasing energy
Metabolic pathways are interconnected series of reactions that regulate metabolic processes and energy flow in cells
Biomolecules Amino Acids
Carbohydrate Metabolism
Carbohydrate metabolism is the process by which carbohydrates are synthesized, broken down, and converted into energy
Glycolysis is the initial step in carbohydrate metabolism, converting glucose into pyruvate
Aerobic respiration occurs in the presence of oxygen, where pyruvate is further metabolized in the citric acid cycle and electron transport chain
Anaerobic respiration occurs in the absence of oxygen, resulting in the conversion of pyruvate into lactate or ethanol
Biomolecules Amino Acids
Lipid Metabolism
Lipid metabolism involves the synthesis, breakdown, and utilization of lipids in the body
Fatty acids undergo beta-oxidation, which breaks them down into acetyl-CoA to generate energy
Lipogenesis is the process of synthesizing fatty acids and triglycerides from acetyl-CoA in the liver
Lipids are stored in adipose tissue and can be hydrolyzed to release fatty acids for energy production
Biomolecules Amino Acids
Nucleic Acid Metabolism
Nucleic acid metabolism involves the biosynthesis and degradation of nucleotides, the building blocks of nucleic acids
Purines (adenine and guanine) and pyrimidines (cytosine, thymine/uracil) are synthesized through complex biochemical pathways
Nucleotides are essential for DNA and RNA synthesis, energy transfer (ATP), and signaling molecules (cyclic AMP)
Nucleotide catabolism involves the breakdown of nucleotides into nucleosides and then individual nitrogenous bases
Biomolecules Amino Acids
Biochemical Pathways
Biochemical pathways refer to the interconnected series of chemical reactions that occur within an organism
They are essential for the efficient utilization of nutrients, energy production, and maintenance of cellular homeostasis
Examples of biochemical pathways include glycolysis, the citric acid cycle, electron transport chain, and oxidative phosphorylation
Understanding these pathways is crucial for comprehending the overall functioning of biochemical systems in living organisms