Application Of Biotechnology In Medicine Vaccine Development
Application of Biotechnology in Medicine - Vaccine Development
Introduction to vaccines
Importance of vaccines in medicine
Traditional methods of vaccine production
Biotechnological approaches to vaccine development
The role of genetic engineering in vaccine production
Application Of Biotechnology In Medicine Vaccine Development
Traditional Methods of Vaccine Production
Attenuated (weakened) pathogen
Inactivated (killed) pathogen
Toxoids
Subunit vaccines
Conjugate vaccines
Application Of Biotechnology In Medicine Vaccine Development
Biotechnological Approaches to Vaccine Development
Recombinant DNA technology
DNA vaccination
Viral vector-based vaccines
Synthetic peptide vaccines
Virus-like particle (VLP) vaccines
Application Of Biotechnology In Medicine Vaccine Development
Recombinant DNA Technology
Introduction to recombinant DNA technology
Cloning and expression of antigenic proteins
Examples of recombinant vaccines - Hepatitis B, Human Papillomavirus (HPV)
Application Of Biotechnology In Medicine Vaccine Development
DNA Vaccination
Introduction to DNA vaccination
Mechanism of action
Steps involved in DNA vaccination
Advantages and limitations
Example - DNA vaccines against COVID-19
Application Of Biotechnology In Medicine Vaccine Development
Viral Vector-Based Vaccines
Introduction to viral vector-based vaccines
Types of viral vectors used
Mechanism of action
Examples - Adenovirus-based vaccines, mRNA vaccines
Application Of Biotechnology In Medicine Vaccine Development
Synthetic Peptide Vaccines
Introduction to synthetic peptide vaccines
Design and synthesis of peptide antigens
Mechanism of action
Examples - Peptide-based vaccines for influenza, cancer
Application Of Biotechnology In Medicine Vaccine Development
Virus-Like Particle (VLP) Vaccines
Introduction to virus-like particle (VLP) vaccines
Production of VLPs using recombinant DNA technology
Advantages of VLP vaccines
Examples - Human papillomavirus (HPV) vaccines, Hepatitis B vaccines
Application Of Biotechnology In Medicine Vaccine Development
Role of Genetic Engineering in Vaccine Production
Genetic modification of microorganisms
Production of antigenic proteins using genetic engineering
Advantages of genetic engineering in vaccine production
Examples - Genetically modified bacteria for vaccine production
Application Of Biotechnology In Medicine Vaccine Development
Conclusion
Recap of the discussed topics
Importance of biotechnological approaches in vaccine development
Future prospects in vaccine research and development
Q&A session
Application Of Biotechnology In Medicine Vaccine Development
Biotechnological Approaches to Vaccine Development (continued)
Reverse vaccinology
Virus-vectored immunoprophylaxis (VIP)
RNA-based vaccines
T-cell-based vaccines
Plant-based vaccines
Application Of Biotechnology In Medicine Vaccine Development
Reverse Vaccinology
Introduction to reverse vaccinology
Genomic analysis of pathogens
Identification of potential antigenic proteins
Production of recombinant vaccines
Example - Meningococcal B vaccine (Bexsero)
Application Of Biotechnology In Medicine Vaccine Development
Virus-Vectored Immunoprophylaxis (VIP)
Introduction to VIP
Use of viral vectors to deliver protective antigen genes
Mechanism of action
Examples - HIV VIP, Ebola VIP
Application Of Biotechnology In Medicine Vaccine Development
RNA-Based Vaccines
Introduction to RNA-based vaccines
Types of RNA vaccines - mRNA, self-amplifying RNA
Mechanism of action
Advantages and limitations
Examples - Pfizer-BioNTech and Moderna COVID-19 vaccines
Application Of Biotechnology In Medicine Vaccine Development
T-Cell-Based Vaccines
Introduction to T-cell-based vaccines
Importance of T-cell immunity in vaccine development
Use of peptide antigens to stimulate T-cell response
Mechanism of action
Examples - Cancer vaccines, HIV vaccines
Application Of Biotechnology In Medicine Vaccine Development
Plant-Based Vaccines
Introduction to plant-based vaccines
Genetic modification of plants for vaccine production
Advantages and limitations
Examples - Edible vaccines, Plant-derived influenza vaccine
Application Of Biotechnology In Medicine Vaccine Development
Importance of Vaccine Development
Control and prevention of infectious diseases
Immunization programs and public health
Eradication of diseases (e.g., smallpox)
Role in global health equity
Pandemic preparedness
Application Of Biotechnology In Medicine Vaccine Development
Challenges in Vaccine Development
Identification of suitable antigenic targets
Safety and efficacy concerns
Manufacturing and scalability
Regulatory approval process
Vaccine hesitancy and misinformation
Application Of Biotechnology In Medicine Vaccine Development
Recent Advances in Vaccine Development
mRNA-based vaccines against COVID-19
Universal influenza vaccines
Therapeutic vaccines for cancer
AI-driven vaccine design
Needle-free vaccine delivery technologies
Application Of Biotechnology In Medicine Vaccine Development
Conclusion
Recap of the discussed topics
Significance of biotechnological approaches in vaccine development
Importance of vaccination in disease prevention
Future directions and challenges in vaccine research
Q&A session
Application Of Biotechnology In Medicine Vaccine Development
Different Types of Vaccines
Live attenuated vaccines
Inactivated vaccines
Subunit vaccines
Toxoid vaccines
Conjugate vaccines
Application Of Biotechnology In Medicine Vaccine Development
Live Attenuated Vaccines
Introduction to live attenuated vaccines
Weakening of pathogenic organisms
Ability to replicate and induce immune response
Examples - Measles, Mumps, Rubella (MMR)
Application Of Biotechnology In Medicine Vaccine Development
Inactivated Vaccines
Introduction to inactivated vaccines
Killing of pathogenic organisms
Retaining antigenicity for immune response
Examples - Polio, Hepatitis A
Application Of Biotechnology In Medicine Vaccine Development
Subunit Vaccines
Introduction to subunit vaccines
Use of purified antigenic components
Elimination of unnecessary components
Examples - Hepatitis B, Human papillomavirus (HPV)
Application Of Biotechnology In Medicine Vaccine Development
Toxoid Vaccines
Introduction to toxoid vaccines
Inactivation of toxins produced by bacteria
Retaining immunogenicity of toxins
Examples - Diphtheria, Tetanus
Application Of Biotechnology In Medicine Vaccine Development
Conjugate Vaccines
Introduction to conjugate vaccines
Linking of bacterial polysaccharides with carrier proteins
Enhancing immune response (especially in infants)
Examples - Haemophilus influenzae type B, Pneumococcal vaccines
Application Of Biotechnology In Medicine Vaccine Development
Vaccine Development Process
Target identification and antigen selection
Development of vaccine candidates
Preclinical testing in animals
Clinical trials (Phase I, II, III)
Regulatory approval and post-marketing surveillance
Application Of Biotechnology In Medicine Vaccine Development
Clinical Trials in Vaccine Development
Phase I
: Safety and dosage determination
Phase II
: Immunogenicity and efficacy assessment
Phase III
: Large-scale trials for verification
Monitoring for adverse effects and long-term safety
Ethics and informed consent
Application Of Biotechnology In Medicine Vaccine Development
Regulatory Approval and Post-Marketing Surveillance
Review by regulatory authorities (e.g., FDA, EMA)
Evaluation of safety and efficacy data
Manufacturing quality control
Vaccine distribution and administration
Monitoring of adverse events and effectiveness
Application Of Biotechnology In Medicine Vaccine Development
Vaccine Hesitancy and Misinformation
Definition and causes of vaccine hesitancy
Impact of misinformation and myths
Strategies to address vaccine hesitancy
Public education and awareness campaigns
Importance of trust and communication with healthcare providers