<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-1-primary"><primary style="font-size:24px"> Slide 1 </primary></h3> <hr> <main> <ul> <li><strong>Topic</strong>: Application of Biotechnology in Agriculture</li> <li><strong>Subtopic</strong>: Expression Cassette</li> </ul> </main> <hr> <footer style = "font-size: 18px"> $\rightarrow$ $\rightarrow$ <span style = "color:blue"> Slide 1 </span> $\rightarrow$ Slide 2 $\rightarrow$ Slide 3 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-1"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-2-primary"><primary style="font-size:24px"> Slide 2 </primary></h3> <hr> <main> <ul> <li>Introduction to Biotechnology in Agriculture</li> <li>Definition and importance of biotechnology in agriculture</li> </ul> </main> <hr> <footer style = "font-size: 18px"> $\rightarrow$ Slide 1 $\rightarrow$ <span style = "color:blue"> Slide 2 </span> $\rightarrow$ Slide 3 $\rightarrow$ Slide 4 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-2"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-3-primary"><primary style="font-size:24px"> Slide 3 </primary></h3> <hr> <main> <ul> <li>Recombinant DNA Technology in Agriculture</li> <li>Introduction to recombinant DNA technology</li> <li>Importance and applications in agriculture</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 1 $\rightarrow$ Slide 2 $\rightarrow$ <span style = "color:blue"> Slide 3 </span> $\rightarrow$ Slide 4 $\rightarrow$ Slide 5 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-3"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-4-primary"><primary style="font-size:24px"> Slide 4 </primary></h3> <hr> <main> <ul> <li>Definition and Components of Expression Cassette</li> <li><strong>Promoter</strong>: Definition and role in gene expression</li> <li><strong>Multiple cloning site (MCS)</strong>: Explanation and significance</li> <li><strong>Gene of interest</strong>: Explanation and its role in desired trait expression</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 2 $\rightarrow$ Slide 3 $\rightarrow$ <span style = "color:blue"> Slide 4 </span> $\rightarrow$ Slide 5 $\rightarrow$ Slide 6 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-4"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-5-primary"><primary style="font-size:24px"> Slide 5 </primary></h3> <hr> <main> <ul> <li>Structure and Function of Expression Cassette</li> <li>Overview of the structure of expression cassette</li> <li>Importance of each component in achieving desired gene expression</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 3 $\rightarrow$ Slide 4 $\rightarrow$ <span style = "color:blue"> Slide 5 </span> $\rightarrow$ Slide 6 $\rightarrow$ Slide 7 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-5"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-6-primary"><primary style="font-size:24px"> Slide 6 </primary></h3> <hr> <main> <ul> <li>Steps involved in Expression Cassette Design</li> <li>Selection of promoter based on target tissue and desired expression level</li> <li>Cloning of the gene of interest into the multiple cloning site</li> <li>Design and optimization of the terminator sequence</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 4 $\rightarrow$ Slide 5 $\rightarrow$ <span style = "color:blue"> Slide 6 </span> $\rightarrow$ Slide 7 $\rightarrow$ Slide 8 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-6"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-7-primary"><primary style="font-size:24px"> Slide 7 </primary></h3> <hr> <main> <ul> <li>Transformation and Gene Expression</li> <li>Overview of transformation methods in plants</li> <li>Factors affecting successful transformation</li> <li>Importance of expression cassette for gene expression in transformed plants</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 5 $\rightarrow$ Slide 6 $\rightarrow$ <span style = "color:blue"> Slide 7 </span> $\rightarrow$ Slide 8 $\rightarrow$ Slide 9 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-7"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-8-primary"><primary style="font-size:24px"> Slide 8 </primary></h3> <hr> <main> <ul> <li>Applications of Expression Cassette in Agriculture</li> <li>Development of genetically modified (GM) crops with desired traits</li> <li>Improved crop yield, stress tolerance, and disease resistance</li> <li>Enhancement of nutritional quality and shelf life of agricultural produce</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 6 $\rightarrow$ Slide 7 $\rightarrow$ <span style = "color:blue"> Slide 8 </span> $\rightarrow$ Slide 9 $\rightarrow$ Slide 10 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-8"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-9-primary"><primary style="font-size:24px"> Slide 9 </primary></h3> <hr> <main> <ul> <li>Case Studies Involving Expression Cassette in Agricultural Biotechnology</li> <li><strong>Bt cotton</strong>: Expression cassette for insect-resistance</li> <li><strong>Golden Rice</strong>: Expression cassette for increased vitamin A content</li> <li><strong>GM crops with herbicide tolerance</strong>: Expression cassette for weed management</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 7 $\rightarrow$ Slide 8 $\rightarrow$ <span style = "color:blue"> Slide 9 </span> $\rightarrow$ Slide 10 $\rightarrow$ Slide 11 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-9"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-10-primary"><primary style="font-size:24px"> Slide 10 </primary></h3> <hr> <main> <ul> <li>Impact and Implications of Expression Cassette in Agriculture</li> <li>Benefits and risks associated with genetically modified organisms (GMOs)</li> <li>Ethical and environmental considerations of GM crops</li> <li>Regulation and public acceptance of GM crops</li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 8 $\rightarrow$ Slide 9 $\rightarrow$ <span style = "color:blue"> Slide 10 </span> $\rightarrow$ Slide 11 $\rightarrow$ Slide 12 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-10"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-11-primary"><primary style="font-size:24px"> Slide 11 </primary></h3> <hr> <main> <ul> <li>Factors Influencing Gene Expression <ul> <li>Environmental factors such as temperature, light, and humidity</li> <li>Developmental stage of the organism</li> <li>Presence of specific regulatory proteins</li> <li>Epigenetic modifications</li> <li>Gene copy number and integration site</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 9 $\rightarrow$ Slide 10 $\rightarrow$ <span style = "color:blue"> Slide 11 </span> $\rightarrow$ Slide 12 $\rightarrow$ Slide 13 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-11"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-12-primary"><primary style="font-size:24px"> Slide 12 </primary></h3> <hr> <main> <ul> <li>Gene Silencing and RNA Interference (RNAi) <ul> <li>Definition and mechanism of gene silencing</li> <li>Importance of gene silencing in regulating gene expression</li> <li>RNAi as a tool to study gene function and control gene expression</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 10 $\rightarrow$ Slide 11 $\rightarrow$ <span style = "color:blue"> Slide 12 </span> $\rightarrow$ Slide 13 $\rightarrow$ Slide 14 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-12"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-13-primary"><primary style="font-size:24px"> Slide 13 </primary></h3> <hr> <main> <ul> <li>Strategies to Enhance Gene Expression <ul> <li>Modifying the promoter sequence to increase gene expression</li> <li>Using enhancer elements to enhance transcription</li> <li>Optimizing codon usage for efficient translation</li> <li>Modifying the 5’ and 3’ untranslated regions (UTRs) to enhance stability and translation efficiency</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 11 $\rightarrow$ Slide 12 $\rightarrow$ <span style = "color:blue"> Slide 13 </span> $\rightarrow$ Slide 14 $\rightarrow$ Slide 15 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-13"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-14-primary"><primary style="font-size:24px"> Slide 14 </primary></h3> <hr> <main> <ul> <li>Expression Cassette Design for Crop Improvement <ul> <li>Introduction to crop improvement through biotechnology</li> <li>Selection of suitable promoter for desired gene expression</li> <li>Gene stacking to introduce multiple traits in a single plant</li> <li>Designing expression cassettes for crop traits like disease resistance, herbicide tolerance, and improved yield</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 12 $\rightarrow$ Slide 13 $\rightarrow$ <span style = "color:blue"> Slide 14 </span> $\rightarrow$ Slide 15 $\rightarrow$ Slide 16 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-14"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-15-primary"><primary style="font-size:24px"> Slide 15 </primary></h3> <hr> <main> <ul> <li>Challenges and Limitations of Expression Cassette Technology <ul> <li>Potential effects on biodiversity and ecosystem</li> <li>Concerns about unintended gene flow and invasiveness</li> <li>Development of resistance in pests or pathogens</li> <li>Ethical and social considerations related to genetic modification</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 13 $\rightarrow$ Slide 14 $\rightarrow$ <span style = "color:blue"> Slide 15 </span> $\rightarrow$ Slide 16 $\rightarrow$ Slide 17 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-15"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-16-primary"><primary style="font-size:24px"> Slide 16 </primary></h3> <hr> <main> <ul> <li>Regulation and Safety of GM Crops <ul> <li>Overview of regulatory agencies and their role in GM crop approval</li> <li>Safety assessments for GM crops</li> <li>Labeling and traceability requirements</li> <li>Public perception and acceptance of GM crops</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 14 $\rightarrow$ Slide 15 $\rightarrow$ <span style = "color:blue"> Slide 16 </span> $\rightarrow$ Slide 17 $\rightarrow$ Slide 18 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-16"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-17-primary"><primary style="font-size:24px"> Slide 17 </primary></h3> <hr> <main> <ul> <li>Advancements in Biotechnology and Future Directions <ul> <li>CRISPR-Cas9 gene editing technology and its potential applications</li> <li>Synthetic biology and the ability to design entirely new genetic systems</li> <li>Precision breeding and genetics-informed crop improvement</li> <li>Sustainable and eco-friendly biotechnology approaches in agriculture</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 15 $\rightarrow$ Slide 16 $\rightarrow$ <span style = "color:blue"> Slide 17 </span> $\rightarrow$ Slide 18 $\rightarrow$ Slide 19 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-17"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-18-primary"><primary style="font-size:24px"> Slide 18 </primary></h3> <hr> <main> <ul> <li>Conclusion and Summary <ul> <li>Recapitulation of the importance of expression cassette in biotechnology</li> <li>Key applications and impacts of expression cassette in agriculture</li> <li>Future prospects and challenges in the field of agricultural biotechnology</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 16 $\rightarrow$ Slide 17 $\rightarrow$ <span style = "color:blue"> Slide 18 </span> $\rightarrow$ Slide 19 $\rightarrow$ Slide 20 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-18"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-19-primary"><primary style="font-size:24px"> Slide 19 </primary></h3> <hr> <main> <ul> <li>References <ul> <li>List of references and resources used for the lecture</li> <li>Books, research papers, and online sources for further reading</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 17 $\rightarrow$ Slide 18 $\rightarrow$ <span style = "color:blue"> Slide 19 </span> $\rightarrow$ Slide 20 $\rightarrow$ Slide 21 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-19"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-20-primary"><primary style="font-size:24px"> Slide 20 </primary></h3> <hr> <main> <ul> <li>Questions for Discussion <ul> <li>Open-ended questions to facilitate class discussion and critical thinking</li> <li>Sample questions: <ul> <li>What are the advantages and disadvantages of using expression cassette technology in agriculture?</li> <li>How can expression cassette design be optimized for achieving desired gene expression in plants?</li> <li>Discuss the ethical and environmental considerations related to the cultivation of GM crops.</li> </ul> </li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 18 $\rightarrow$ Slide 19 $\rightarrow$ <span style = "color:blue"> Slide 20 </span> $\rightarrow$ Slide 21 $\rightarrow$ Slide 22 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-20"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-21-primary"><primary style="font-size:24px"> Slide 21 </primary></h3> <hr> <main> <ul> <li>Techniques for Expression Cassette Delivery <ul> <li>Agrobacterium-mediated gene transfer</li> <li>Particle bombardment (biolistics)</li> <li>Microinjection</li> <li>Electroporation</li> <li>Viral vectors</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 19 $\rightarrow$ Slide 20 $\rightarrow$ <span style = "color:blue"> Slide 21 </span> $\rightarrow$ Slide 22 $\rightarrow$ Slide 23 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-21"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-22-primary"><primary style="font-size:24px"> Slide 22 </primary></h3> <hr> <main> <ul> <li>Factors Affecting Gene Expression in Transgenic Plants <ul> <li>Position effect</li> <li>Gene silencing</li> <li>Epigenetic modifications</li> <li>Copy number variation</li> <li>Insertion site effects</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 20 $\rightarrow$ Slide 21 $\rightarrow$ <span style = "color:blue"> Slide 22 </span> $\rightarrow$ Slide 23 $\rightarrow$ Slide 24 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-22"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-23-primary"><primary style="font-size:24px"> Slide 23 </primary></h3> <hr> <main> <ul> <li>Methods to Analyze Gene Expression in Transgenic Plants <ul> <li>PCR-based techniques (RT-PCR, qPCR)</li> <li>Northern blotting</li> <li>In situ hybridization</li> <li>Reporter genes (e.g., GUS, GFP)</li> <li>Proteomics and metabolomics</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 21 $\rightarrow$ Slide 22 $\rightarrow$ <span style = "color:blue"> Slide 23 </span> $\rightarrow$ Slide 24 $\rightarrow$ Slide 25 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-23"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-24-primary"><primary style="font-size:24px"> Slide 24 </primary></h3> <hr> <main> <ul> <li>Gene Expression Monitoring Tools <ul> <li>DNA microarrays</li> <li>RNA-seq</li> <li>ChIP-seq</li> <li>Reporter gene assays</li> <li>Immunohistochemistry</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 22 $\rightarrow$ Slide 23 $\rightarrow$ <span style = "color:blue"> Slide 24 </span> $\rightarrow$ Slide 25 $\rightarrow$ Slide 26 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-24"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-25-primary"><primary style="font-size:24px"> Slide 25 </primary></h3> <hr> <main> <ul> <li>Examples of Expression Cassette Applications <ul> <li>Insect-resistant crops (e.g., Bt cotton, Bt corn)</li> <li>Herbicide-tolerant crops (e.g., Roundup Ready soybeans)</li> <li>Disease-resistant crops (e.g., virus-resistant papaya)</li> <li>Nutritionally enhanced crops (e.g., Golden Rice)</li> <li>Biofortification of staple crops</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 23 $\rightarrow$ Slide 24 $\rightarrow$ <span style = "color:blue"> Slide 25 </span> $\rightarrow$ Slide 26 $\rightarrow$ Slide 27 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-25"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-26-primary"><primary style="font-size:24px"> Slide 26 </primary></h3> <hr> <main> <ul> <li>Environmental Impact of GM Crops <ul> <li>Potential effects on non-target organisms</li> <li>Gene flow and hybridization with wild relatives</li> <li>Impact on biodiversity and ecosystem services</li> <li>Pest resistance development</li> <li>Soil and water contamination risks</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 24 $\rightarrow$ Slide 25 $\rightarrow$ <span style = "color:blue"> Slide 26 </span> $\rightarrow$ Slide 27 $\rightarrow$ Slide 28 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-26"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-27-primary"><primary style="font-size:24px"> Slide 27 </primary></h3> <hr> <main> <ul> <li>Regulations and Safety Assessments <ul> <li>Regulatory agencies for GM crop approvals</li> <li>Pre-market safety assessments</li> <li>Allergenicity and toxicity testing</li> <li>Post-market monitoring and labeling requirements</li> <li>International agreements and guidelines</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 25 $\rightarrow$ Slide 26 $\rightarrow$ <span style = "color:blue"> Slide 27 </span> $\rightarrow$ Slide 28 $\rightarrow$ Slide 29 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-27"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-28-primary"><primary style="font-size:24px"> Slide 28 </primary></h3> <hr> <main> <ul> <li>Public Perception and Acceptance of GM Crops <ul> <li>Factors influencing public opinion</li> <li>Trust in regulatory systems and industry</li> <li>Communication and education efforts</li> <li>Public participation in decision-making processes</li> <li>Consumer attitudes towards GM food products</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 26 $\rightarrow$ Slide 27 $\rightarrow$ <span style = "color:blue"> Slide 28 </span> $\rightarrow$ Slide 29 $\rightarrow$ Slide 30 </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-28"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-29-primary"><primary style="font-size:24px"> Slide 29 </primary></h3> <hr> <main> <ul> <li>Future Directions in Biotechnology and Agriculture <ul> <li>Genome editing technologies (e.g., CRISPR-Cas9)</li> <li>Synthetic biology and bioengineered organisms</li> <li>Precision agriculture and data-driven farming</li> <li>Climate-smart crop improvement strategies</li> <li>Plant-based biopharmaceutical production</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 27 $\rightarrow$ Slide 28 $\rightarrow$ <span style = "color:blue"> Slide 29 </span> $\rightarrow$ Slide 30 $\rightarrow$ </footer>

<h3 id="success-stylefont-size25px-application-of-biotechnology-in-agriculture-expression-cassette-success-29"><Success style="font-size:25px"> Application Of Biotechnology In Agriculture Expression Cassette </Success></h3> <h3 id="primary-stylefont-size24px-slide-30-primary"><primary style="font-size:24px"> Slide 30 </primary></h3> <hr> <main> <ul> <li>Summary and Key Takeaways <ul> <li>Expression cassette is a crucial tool in biotechnology for gene expression in transgenic plants.</li> <li>It consists of a promoter, gene of interest, and terminator sequence.</li> <li>Expression cassette design involves selecting appropriate components and optimizing gene expression.</li> <li>GM crops developed using expression cassettes have various applications in agriculture.</li> <li>Environmental impact, regulations, and public perception are important considerations in GM crop cultivation.</li> </ul> </li> </ul> </main> <hr> <footer style = "font-size: 18px">Slide 28 $\rightarrow$ Slide 29 $\rightarrow$ <span style = "color:blue"> Slide 30 </span> $\rightarrow$ $\rightarrow$ </footer>