cx-ncert-theory-class12-chap05-molecular-basis-of-inheritance-part-7-en

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<h6 id="primaryhuman-genome-projectprimary"><Primary>Human genome project</Primary></h6>
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<p>In other words, genetic make-up of an organism or an individual lies in The DNA sequences. If two individuals differ, then their DNA sequences should also be different, at least at some places.</p>
<p>These assumptions led to the quest of finding out the complete DNA sequence of human genome.</p>
<p>With the establishment of genetic engineering techniques where it was possible to isolate and clone any piece of DNA and availability of simple and fast techniques for determining DNA sequences, a very ambitious project of sequencing human genome was launched in the year 1990</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<p>Human genome project (HGP) was called a mega project. You can imagine the magnitude and the requirements for the project if we simply define the aims of the project as follows: Human genome is said to have approximately 3 x $10^9$ bp, and if the cost of sequencing required is US $ 3 per bp (the estimated cost in the beginning), the total estimated cost of the project would be approximately
9 billion US dollars.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-2"><Primary>Human genome project</Primary></h6>
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<p>Further, if the obtained sequences were to be stored in typed form in books, and if each page of the book contained 1000 letters and each book contained 1000 pages, then 3300 such books would be required to store the information of DNA sequence from a single human cell.</p>
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<li>The enormous amount of data expected to be generated also necessitated the use of high speed computational devices for data storage and retrieval, and analysis. HGP was closely associated with the rapid development of a new area in biology called Bioinformatics.</li>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<p><strong>Goals of HGP</strong></p>
<p>Some of the important goals of HGP were as follows:</p>
<p>(i) Identify all the approximately 20,000-25,000 genes in human DNA;</p>
<p>(ii) Determine the sequences of the 3 billion chemical base pairs that make up human DNA;</p>
<p>(iiii) Store this information in databases;</p>
<p>(iv) Improve tools for data analysis;</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-4"><Primary>Human genome project</Primary></h6>
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<p>(v) Transfer related technologies to other sectors, such as industries;</p>
<p>(vi) Address the ethical, legal, and social issues (ELSI) that may arise from the project.</p>
<p>The Human genome project was a 13-year project coordinated by the U.S. Department of Energy and the National Institute of Health. During the early years of the HGP, the Wellcome Trust (U.K.) became a major partner; additional contributions came from Japan, France, Germany, China and others. The project was completed in 2003.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-5"><Primary>Human genome project</Primary></h6>
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<p>Knowledge about the effects of DNA variations among individuals can lead to revolutionary new ways to diagnose, treat and someday prevent the thousands of disorders that affect human beings. Besides providing clues to understanding human biology, learning about non-human organisms DNA sequences can lead to an understanding of their natural capabilities that can be applied toward solving challenges in health care, agriculture, energy production, environmental remediation.</p>
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<li>Many non-human model organisms, such as bacteria, yeast, Caenorhabditis elegans (a free living non-pathogenic nematode), Drosophila (the fruit fly), plants (rice and Arabidopsis), etc., have also been sequenced.</li>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

<h6 id="successmolecular-basis-of-inheritancesuccess-6"><Success>Molecular basis of inheritance</Success></h6>
<h6 id="primaryhuman-genome-projectprimary-6"><Primary>Human genome project</Primary></h6>
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<p><strong>Methodologies :</strong> The methods involved two major approaches. One approach focused on identifying all the genes that are expressed as RNA (referred to as Expressed Sequence Tags (ESTs).</p>
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<li>The other took the blind approach of simply sequencing the whole set of genome that contained all the coding and non-coding sequence, and later assigning different regions in the sequence with functions (a term referred to as Sequence Annotation).</li>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

<h6 id="successmolecular-basis-of-inheritancesuccess-7"><Success>Molecular basis of inheritance</Success></h6>
<h6 id="primaryhuman-genome-projectprimary-7"><Primary>Human genome project</Primary></h6>
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<p>For sequencing, the total DNA from a cell is isolated and converted into random fragments of relatively smaller sizes (recall DNA is a very long polymer, and there are technical limitations in sequencing very long pieces of DNA) and cloned in suitable host using specialised vectors. The cloning resulted into amplification of each piece of DNA fragment so that it subsequently could be sequenced with ease. The commonly used hosts were bacteria and yeast, and the vectors were called as BAC (bacterial artificial chromosomes), and YAC (yeast artificial chromosomes).</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-8"><Primary>Human genome project</Primary></h6>
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<p>The fragments were sequenced using automated DNA sequencers that worked on the principle of a method developed by Frederick Sanger. (Remember, Sanger is also credited for developing method for determination of amino acid sequences in proteins). These sequences were then arranged based on some overlapping regions present in them. This required generation of overlapping fragments for sequencing. Alignment of these sequences was humanly not possible. Therefore, specialised computer based programs were developed (Figure 5.15).</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

<h6 id="successmolecular-basis-of-inheritancesuccess-10"><Success>Molecular basis of inheritance</Success></h6>
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<p>These sequences were subsequently annotated and were assigned to each chromosome. The sequence of chromosome 1 was completed only in May 2006 (this was the last of the 24 human chromosomes – 22
autosomes and X and Y – to be sequenced). Another challenging task was assigning the genetic and physical maps on the genome.</p>
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<li>This was generated using information on polymorphism of restriction endonuclease recognition sites, and some repetitive DNA sequences known as microsatellites (one of the applications of polymorphism in repetitive DNA sequences shall be explained in next section of DNA fingerprinting).</li>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-11"><Primary>Human genome project</Primary></h6>
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<p><strong>Salient Features of Human Genome</strong></p>
<p>Some of the salient observations drawn from Human genome project are as follows:</p>
<p>(i) The human genome contains 3164.7 million bp.</p>
<p>(ii) The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<p>(iii) The total number of genes is estimated at 30,000–much lower than previous estimates of 80,000 to 1,40,000 genes. Almost all (99.9 per cent) nucleotide bases are exactly the same in all people.</p>
<p>(iv) The functions are unknown for over 50 per cent of the discovered genes.</p>
<p>(v) Less than 2 per cent of the genome codes for proteins.</p>
<p>(vi) Repeated sequences make up very large portion of the human genome.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<p>(vii) Repetitive sequences are stretches of DNA sequences that are repeated many times, sometimes hundred to thousand times. They are thought to have no direct coding functions, but they shed light
on chromosome structure, dynamics and evolution.</p>
<p>(viii) Chromosome 1 has most genes (2968), and the Y has the fewest (231).</p>
<p>(ix) Scientists have identified about 1.4 million locations where singlebase DNA differences (SNPs – single nucleotide polymorphism, pronounced as ‘snips’) occur in humans. This information promises
to revolutionise the processes of finding chromosomal locations for disease-associated sequences and tracing human history.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

<h6 id="successmolecular-basis-of-inheritancesuccess-14"><Success>Molecular basis of inheritance</Success></h6>
<h6 id="primaryhuman-genome-projectprimary-14"><Primary>Human genome project</Primary></h6>
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<p><strong>Applications and Future Challenges</strong></p>
<p>Deriving meaningful knowledge from The DNA sequences will define research through the coming decades leading to our understanding of biological systems. This enormous task will require the expertise and creativity of tens of thousands of scientists from varied disciplines in both the public and private sectors worldwide.</p>
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<li>One of the greatest impacts of having the HG sequence may well be enabling a radically new approach to biological research. In the past, researchers studied one or a few genes at a time.</li>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>

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<h6 id="primaryhuman-genome-projectprimary-15"><Primary>Human genome project</Primary></h6>
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<p>With whole-genome sequences and new high-throughput technologies, we can approach questions systematically and on a much broader scale. They can study all the genes in a genome, for example, all the transcripts in a particular tissue or organ or tumor, or how tens of thousands of genes and proteins work together in interconnected networks to orchestrate the chemistry of life.</p>
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<footer style="font-size:18px">Introduction$\rightarrow$ The DNA$\rightarrow$ The Search for Genetic Material$\rightarrow$ RNA World$\rightarrow$ Replication$\rightarrow$ Transcription$\rightarrow$ Genetic code$\rightarrow$ Translation$\rightarrow$ Regulation of gene expression$\rightarrow$ <span style="color:blue">Human genome project</span>$\rightarrow$ DNA fingerprinting</footer>