Ques. In view of the current food crisis, it is said, that we need another green revolution.
Ans. Major limitations of the earlier green revolution are as follows
(i) Generally undesired characters also breed along with desired ones
Ans. to be continued
(ii) Regular use of fertilisers affected both soil fertility and quality.
(iii) Extensive use of pesticides/insecticides/weedicides resulted in harmful effects on the natural components of the ecosystem.
Ques. Expand GMO.
Ans. GMO stands for Genetically Modified Organism that contains one or more genes from an entirely different species and is genetically altered during molecular genetics such as gene cloning, protein engineering etc.
Ques. Differentiate between diagnostics and therapeutics.
Ans. A diagnostic technique helps us to identify a disease, e.g., ELISA for HIV.
Ques. Give the full form of ELISA.
Ans. ELISA stands for Enzyme Linked Immuno Sorbent Assay.
It is used for the detection of (HIV) AIDS disease.
ELISA is based on the principle of antigen-antibody interaction.
Infection by pathogen can be detected by the presence of antigens (proteins, glycoprotieins, etc.) or by detecting the antibodies synthesised by the host against the pathogen.
Ques. Can a disease be detected before its symtoms appear? Explain the principle involved.
Ans. When the symptoms of the disease are not yet visible,the pathogen concentration is very low so, the detection by conventional diagnostic tests is very difficult.
Ques. Write a short note on biopiracy highlighting the exploitation of developing countries by the developed countries.
Ans. Biopiracy is the term used to refer the use of bio-resources by the multinational companies and other organisations without proper authorisation from the countries and from the people concerned without compensatory payment.
In contrast, the developing and the underdeveloped world is rich in biodiversity and traditional knowledge related to bio-resources.
So, sometimes industrialised nations tend to exploit the resources or biodiversity of developing nations, for their own advantages.
Ques. Many proteins are secreted in their inactive form.
Ans. Many proteins including certain toxins are secreted in their inactive form.
Ques. While creating genetically modified organisms, genetic barriers are not respected.
Ans. Genetic modification of organisms can have unpredictable results when such organisms are introduced into the ecosystem.
Ques. Why has the Indian parliament cleared the second amendment of the country’s patents bill?
Ans. Amendments to the patent bill has empowered India to prevent unauthorised exploitation of our bio-resources and traditional knowledge by other countries.
Ques. Give any two reasons why the patent on Basmati should not have gone to an American Company.
Ans. The patent on Basmati should not have gone to an American Company, because of the following reasons
(i) The Basmati rice variety has been grown in India since time immemorial.
(ii) The new variety of Basmati that got patent rights to an American Company is actually derived from ‘Indian farmers variety’.
Ques. How was insulin obtained before the advent of $r$ DNA technology? What were the problems encountered?
Ans. Insulin is used for diabetes treatment and it was earlier extracted from the pancreas of slaughtered cattle and pigs.
Ques. With respect to understanding diseases, discuss the importance of transgenic animal models.
Ans. Transgenic animals are important in the following fields
(i) They are being used in basic science research to elucidate, the role of genes in the development of diseases like cancer, cystic fibrosis, rheumatoid arthritis and alzheimer’s.
(ii) They are valuable tools in the drug development process itself.
(iii) They can produce medicines or human proteins (insulin, growth hormone, etc.) in large quantities.
(iv) Transgenics can be a source of transplant organs as well.
Ques. Name the first transgenic cow.
Ans. Rosie was the name of first transgenic cow.
Ques. PCR is a useful tool for early diagnosis of an infectious disease.
Ans. PCR is a very sensitive technique which enables the amplification of desired DNA from a limited amount of DNA template.
Ques. What is GEAC and what are its objectives?
Ans. GEAC (Genetic Engineering Approval Committee) is an Indian Government Organisation.
(i) Examine the validity of Genetic Modification (GM) of organism research.
(ii) Inspect the safety of introducing GMO for public services.
Ques. For which variety of Indian rice, the patent was filed by a USA company?
Ans. Indian Basmati rice was crossed with semi-dwarf variety and was claimed as a new variety for which the patent was field by a USA company.
Ques. Discuss the advantages of GMO.
Ans. Advantages of GMO are
(i) GMO food crops have shorter growing cycles, greater resistance to both insects and diseases, higher yields and higher nutritional value.
(ii) GMO animals have increased production and nutritive values, e.g., GM, cows may produce more milk.
(iii) The World Health Organisation or WHO, claims that GMO plants and animals may allow food prices to drop as food sources become more abundant.
Ques. Gene expression can be controlled with the help of RNA.
Ans. RNAi technology is used to block the expression of certain genes and also referred to as gene silencing.
E.g., a nematode Meloidegyne incognitia infects the roots of tobacco plants which reduces the production of tobacco.
It can be prevented by using RNA interference (RNAi) process which is checked by silencing of specific $m R N A$ due to a complementary dsRNA.
Ques. Ignoring our traditional knowledge can be prove costly in the area of biological patenting.
Ans. Human communities have always generated, refined and passed on the knowledge from generation to generation.