Ques. Bt cotton is not
(a) A GM plant (b) Insect resistant (c) A bacterial gene expressing system (d) Resistant to all pesticides
Ans. (d)
Sol. Bt cotton is a genetically modified plant whose genes have been altered by the manipulations to make it insect resistant through the introduction of $B t$ toxin gene.
Sol. to be continued
Ques. C-peptide of human insulin is
(a) A part of mature insulin molecule (b) Responsible for the formation of disulphide bridges (c) Removed during maturation of pro-insulin to insulin (d) Responsible for its biological activity
Thinking Process
Ans. (c)
Sol. The connecting peptide or C-peptide is a short protein containing 31 amino acids.
Ques. GEAC stands for
(a) Genome Engineering Action Committee (b) Ground Environment Action Committee (c) Genetic Engineering Approval Committee (d) Genetic and Environment Approval Committee
Sol. GEAC stands for Genetic Engineering Approval Committee.
Ques. Antitrypsin is
(a) An antacid (b) An enzyme (c) Used to treat arthritis (d) Used to treat emphysema
Sol. $\alpha$-antitrypsin is a protease inhibitor that is produced by the introduction of specific sequence of DNA (or genes) which codes for a particular product in the transgenic animals.
Ques. A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be
(a) A single stranded RNA (b) A single stranded DNA (c) Either RNA or DNA (d) Can be $s s$ DNA but not $s s$ RNA
Sol. A probe is a single stranded DNA or RNA tagged with a radioactive molecule.
Ques. Choose the correct option regarding retrovirus.
(a) A RNA virus that can synthesis DNA during infection (b) A DNA virus that can synthesis RNA during infection (c) A ssDNA virus (d) A dsRNA virus
Ans. (a)
Sol. A retrovirus is a single-stranded RNA virus that stores its nucleic acid in the form of mRNA genome (5’cap and 3’poly A tail).
However, in retroviruses their RNA is reverse-transcribed into DNA, which is integrated into the host cell’s genome (when it becomes a provirus) and then undergoes the usual transcription and translational process to express the genes carried by the virus, i.e.
RNA $\rightarrow$ DNA $\rightarrow$ RNA $\rightarrow$ Polypeptide.
Ques. The site of production of ADA in the body is
(a) Erythrocytes (b) Lymphocytes (c) Blood plasma (d) Osteocytes
Ans. (b)
Sol. ADA gene is responsible for producing the enzyme adenosine deaminase.
Ques. $\mathrm{~A}$ protoxin is
(a) A primitive toxin (b) A denatured toxin (c) Toxin produced by Protozoa (d) Inactive toxin
Sol. A protoxin is an inactive toxin.
Ques. Pathophysiology is the
(a) Study of physiology of pathogen (b) Study of normal physiology of host (c) Study of altered physiology of host (d) None of these
Sol. Pathophysiology is the study of functional changes in the body of the host that occur in response to any disease or injury.
Ques. The trigger for activation of toxin of Bacillus thuringiensis is
(a) Acidic $\mathrm{pH}$ of stomach (b) High temperature (c) Alkaline $\mathrm{pH}$ of gut (d) Mechanical action in the insect gut
Sol. Bt toxins are initially inactive protoxins but after ingestion by the insect these inactive toxin becomes active due to the alkaline $\mathrm{pH}$ of the gut which solublise the crystals.
Ques. Golden rice is
(a) A variety of rice grown along the yellow river in China (b) Long stored rice having yellow colour tint (c) A transgenic rice having gene for $\beta$ - carotene (d) Wild variety of rice with yellow coloured grains
Sol. Golden rice is a genetically modified crop with enhanced nutritional value.
Ques. In RNAi, genes are silenced using
(a) SS DNA (b) $d s$ DNA (c) $d s$ RNA (d) SS RNA
Sol. A nematode Meloidegyne incognitia infects the roots of tobacco plants which reduces the production of tobacco.
Ques. The first clinical gene therapy was done for the treatment of
(a) AIDS (b) Cancer (c) Cystic fibrosis (d) SCID (Severe Combined Immuno Deficiency) resulting from the deficiency of ADA
Sol. The first clinical gene therapy was done for the treatment of SCID (Severe Combined Immuno Deficiency) resulting from the deficiency of ADA.
Ques. ADA is an enzyme which is deficient in a genetic disorder SCID.
(a) Adenosine Deoxy Aminase (b) Adenosine Deaminase (c) Aspartate Deaminase (d) Arginine Deaminase
Sol. ADA is an enzyme which is deficient in a genetic disorder SCID.
Ques. Silencing of a gene could be achieved through the use of
(a) Short interfering RNA (RNAi) only (b) Antisense RNA only (c) Both RNAi and antisense RNA (d) None of these
Sol. Gene silencing could be achieved through various routes including the use of RNAi, antisense RNA, ribozymes, etc.