Biotechnology- Principles and Processes Question 9
9. Match the following enzymes with their functions: [2019, Odisa]
| | Column-I | | Column-II |
| :—: | :—: | :—: | :—: |
| (A) | Restriction
endonuclease | (i) | Joins the DNA
fragments |
| (B) | Restriction
exonuclease
DNA | (ii) | Extends primers
on genomic
template |
| (C) | DNA ligase | (iii) | Cuts DNA at
specific position |
| (D) | Tag polymerase | (iv) | Removes
nucleotides from
the ends of DNA |
Select the correct option from the following:
(a) (A)-(ii), (B)-(iv), (C)-(i), (D)-(iii)
(b) (A)-(iii), (B)-(i), (C)-(iv), (D)-(ii)
(c) (A)-(iii), (B)-(iv), (C)-(i), (D)-(ii)
(d) (A)-(iv), (B)-(iii), (C)-(i), (D)-(ii)
Show Answer
Answer : c
Hints & Solutions
(c) Restriction endonuclease cleaves DNA in to fragments at or near specific recognition sites within the molecule known as restriction sites. Restriction exonucleases cleave off nucleotides one at a time from the $3^{\prime}$ or 5 ’ ends of DNA and RNA chains. DNA ligase facilitates the joining of DNA strands together by catalysing the formation of a phosphodiester bond. Taq polymerase is a thermostable DNA polymerase I named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Chien et al. in 1976. It attaches nucleotides to a DNA template, thereby copying the DNA.
