Biotechnology- Principles and Processes Question 5
5. The sequence that controls the copy number of the linked DNA in the vector, is termed
[2020]
(a) Ori site
(b) Palindromic sequence
(c) Recognition site
(d) Selectable marker
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Answer : a
Hints & Solutions
(a) The correct option is
(a) because ori sequence is responsible for controlling the copy number of the linked DNA in the vector. Ori i.e. origin of replication is responsible for initiation of replication. The high AT-content results in the low thermodynamic stability of the region which accounts for its role in the process of replication initiation. At the AT-rich regions, the initial DNA helix destabilization (opening) is induced by binding an initiator protein to its respective recognition sequences situated nearby.
