Biotechnology- Principles and Processes Question 3
3. Identify the wrong statement with regard to Restriction Enzymes.
[2020]
(a) They cut the strand of DNA at palindromic sites.
(b) They are useful in genetic engineering.
(c) Sticky ends can be joined by using DNA ligases.
(d) Each restriction enzyme functions by inspecting the length of a DNA sequence.
Show Answer
Answer : c
Hints & Solutions
(c) Restriction endonucleases make cuts at specific positions within the DNA. They function by inspecting the length of a DNA sequence. Restriction endonuclease binds to the DNA and cut the two strands of double helix at specific points in their sugarphosphate backbones. They are used in genetic engineering to form recombinant molecules of DNA. DNA ligases join the DNA fragments. The restriction enzyme prevents replication of the phage DNA by cutting it into many pieces. Restriction enzymes were named for their ability to restrict, or limit, the number of strains of bacteriophage that can infect a bacterium.
