Biotechnology- Principles and Processes Question 28
28. The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component (s)?
[2012]
(a) ori-original restriction enzyme
(b) rop-reduced osmotic pressure
(c) Hind III, EcoRI - selectable markers
(d) $a m p^{R}, t^{R}$ - antibiotic resistance genes
Show Answer
Answer : d
Hints & Solutions
(d) In pBR 322 ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid. Hind III, EcoRI- Recoginition sites of Restriction endonucleases $a m p^{R}$ and tet $^{R}$ - They are antibiotic resistant gene part The Rop protein (Repressor of Protein) is involved in stabilizing the interaction between RNA I and RNA II, which in turn prevents the replication of pBR322.
