Biotechnology- Principles and Processes Question 23
23. An analysis of chromosomal DNA using the Southern hybridization technique does not use:
(a) Electrophoresis
(b) Blotting
(c) Autoradiography
(d) PCR
Show Answer
Answer : d
Hints & Solutions
(d) PCR is a technique for enzymatically replicating DNA without using a living organism such as E. coli or Yeast. It is commonly used in medical and biological research labs for a variety of tasks like detection of hereditary diseases, identification of genetic fingerprints etc. Blotting is the transfer of the fragmented DNA sequence to the nitrocellulose membrane or nylon membrane. The process is done by either electroblotting or capillary blotting. The DNA molecule is saturated using a $NaCl$ solution and permanently fixed using either UV radiation or drying.
