Biotechnology- Principles and Processes Question 20

20. Which of the following is a restriction endonuclease?

[2016]

(a) Hind II

(b) Protease

(c) DNase I

(d) RNase

Show Answer

Answer : a

Hints & Solutions

(a) A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Hind II among these is a type of restriction endonuclease. A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them.