Biotechnology- Principles and Processes Question 15
15. The DNA fragments separated on an agarose gel can be visualised after staining with: [2017]
(a) Acetocarmine
(b) Aniline blue
(c) Ethidium bromide
(d) Bromophenol blue
Show Answer
Answer : c
Hints & Solutions
(c) Ethidium bromide ( $EtBr)$ is used to stain the DNA fragments and will appear as orange coloured bands when kept under UV light. Ethidium bromide dye is most commonly used for the detection of DNA/ RNA. It is a DNA intercalating agent, inserting itself into the spaces between the base pairs of the double helix. It possesses UV absorbance maxima at 300 and $360 nm$.
