Steps In Gram Staining
Steps in gram staining
graph TD;
A[Steps in Gram Staining];
A --> B[Step 1: Crystal Violet];
A --> C[Step 2: Iodine Treatment];
A --> D[Step 3: Alcohol Wash];
A --> E[Step 4: Safranin Counterstain];
B --> F[Stains cell wall purple];
C --> G[Binds to crystal violet and forms a complex];
D --> H[Decolorizes cells];
E --> I[Stains decolorized cells pink];
F --> J[All bacteria];
G --> K[All bacteria];
H --> L[Gram-positive bacteria retain color];
H --> M[Gram-negative bacteria lose color];
I --> N[Gram-negative bacteria];
