| Step Number | Krebs Cycle Step | Substrate/Reactants | Products | Enzyme | Energy Gained |
|---|---|---|---|---|---|
| Step 1 | Acetyl-CoA Entry | Acetyl-CoA + Oxaloacetate | Citrate | Citrate Synthase | - |
| Step 2 | Isomerization | Citrate | Isocitrate | Aconitase | - |
| Step 3 | First Oxidation | Isocitrate | Alpha-Ketoglutarate + CO2 + NADH | Isocitrate Dehydrogenase | 1 NADH (per isocitrate) |
| Step 4 | Second Oxidation | Alpha-Ketoglutarate | Succinyl-CoA + CO2 + NADH | Alpha-Ketoglutarate Dehydrogenase | 1 NADH (per alpha-ketoglutarate) |
| Step 5 | Substrate-Level Phosphorylation | Succinyl-CoA | Succinate + GTP (or ATP) | Succinyl-CoA Synthetase | 1 GTP (or ATP) |
| Step 6 | Oxidation and Electron Transfer | Succinate | Fumarate + FADH2 | Succinate Dehydrogenase | 1 FADH2 (per succinate) |
| Step 7 | Hydration | Fumarate | Malate | Fumarase | - |
| Step 8 | Final Oxidation | Malate | Oxaloacetate + NADH | Malate Dehydrogenase | 1 NADH (per malate) |
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